Summary
A novel mutation, FruS localised in the fru operon was obtained. It uncouples expression of the genes determining synthesis of the fructose-specific transport proteins and fructose- l-phosphate kinase. In FruS bacteria the fruA and fruF genes (coding for Enzyme IIfru and FPr, respectively) are constitutive by expressed while fruK (encoding fructose-1-phosphate kinase) remains inducible. In contrast to other mutations, which render expression of the whole fru operon constitutive, the FruS mutation: (1) does not lead to d-xylitol sensitivity; (2) does not inhibit growth on D-lactate, pyruvate and l-alanine; (3) does not decrease phosphoenolpyruvate (PEP) synthase activity.
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Communicated by J. Lengeler
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Bolshakova, T.N., Molchanova, M.L., Erlagaeva, R.S. et al. A novel mutation FruS, altering synthesis of components of the phosphoenolpyruvate: fructose phosphotransferase system in Escherichia coli K12. Molec. Gen. Genet. 232, 394–398 (1992). https://doi.org/10.1007/BF00266242
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DOI: https://doi.org/10.1007/BF00266242