Summary
Restriction fragment length polymorphism analyses of SLA class I genes were performed on 55 Duroc and 24 Hampshire boars from the 1986–87 national performance tests of each breed. Few boars were inbred. Southern blotting and hybridization procedures were performed on genomic DNA isolated from white blood cells by using Pvu II, Bam HI, and Eco RI endonucleases and a swine MHC class I probe. Genetic variability within and between the two breeds was estimated in terms of nucleotide diversity, by using a mathematical analysis based on the different RFLP patterns. The nucleotide diversity calculated within each breed was less than that between the two breeds. The results from the nucleotide diversity analysis suggested that genetic variability was greater in the Duroc breed than in the Hampshire breed. A relatively high level of genetic variability was shown in the class I major histocompatibility complex genes in the pig.
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References
Brown WM (1980) Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis. Proc Natl Acad Sci USA 77:3605–3609
Chardon P, Vaiman M, Renard C, Arnoux B (1978) Pig histocompatibility antigens and β 2-microglobulin. Transplantation 26:107–112
Chardon P, Vaiman M, Kirszenbaum M, Geffrotin C, Renard C, Cohen D (1985) Restriction fragment length polymorphism of the major histocompatibility complex of pig. Immunogenetics 21:161–171
Ehrlich R, Singer DS (1986) Organization and expression of class I MHC genes in the miniature swine. Fed Proc 4:846
Flanagan MP, Jung YC, Rothschild MF, Warner CM (1988) RFLP analysis of SLA class I genotypes in Duroc swine. Immunogenetics 27:465–469
Kaplan N (1983) Statistical analysis of restriction enzyme map data and nucleotide sequence data. In: Wier BS (ed) Statistical analysis of DNA sequence data. Marcel Dekker, New York, pp75–106
Kirszenbaum M, Renard C, Geffrotin C, Chardon P, Vaiman M (1985) Evidence for mapping pig C4 gene(s) within the pig major histocompatibility complex (SLA). Anim Blood Groups Biochem Genet 16:65–68
Lie WR, Rothschild MF, Warner CM (1987) Mapping of C2, Bf and C4 genes to the swine major histocompatibility complex (SLA). J Immunol 139:3388–3395
Lunney JK, Sachs DH (1978) Transplantation in miniature swine. IV. Chemical characterization of MSLA and Ia-like antigens. J Immunol 120:607–612
Lunney JK, Pescovitz D, Sachs DH (1986) The swine major histocompatibility complex: Its structure and function. In: Tumbleson ME (ed) Swine in biomedial research vol. 3. Plenum Press, New York London, pp 1821–1836
Nei M (1975) Molecular population genetics and evolution. North-Holland, Amsterdam Oxford, pp 224–230
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York, pp 106–107
Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273
Nei M, Tajima F (1981) DNA polymorphism detectable by restriction endonucleases. Genetics 97:145–163
Nei M, Tajima F (1983) Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data. Genetics 105:207–217
Nei M, Tajima F, Gojobori T (1984) Classification and measurement of DNA polymorphism. In: Chakravarti A (ed) Human population genetics: The Pittsburgh Symposium. Van Nostrand Reinhold, New York pp 307–330
Satz ML, Wang L-C, Singer DS, Rudikoff S (1985) Structure and expression of two porcine genome clones encoding class I MHC antigens. J Immunol 135:2167–2175
Schreier PH, Bothwell ALM, Muller-Hill B, Baltimore D (1981) Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse. Proc Natl Acad Sci USA 78:4495–4499
Singer DS, Camerini-Otero RD, Satz MI, Osborne B, Sachs D, Rudikoff S (1982) Characterization of a porcine genomic clone encoding a major histocompatibility antigen: Expression in mouse L cells. Proc Natl Acad Sci USA 79:1403–1407
Singer DS, Lifshitz R, Ableson L, Nyrjesy P, Rudikoff S (1983) Specific association of repetitive DNA-sequences with major histocompatibility genes. Mol Cell Biol 3:903–913
Upholt WB (1977) Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res 4:1257–1265
Vaiman M, Hauptman G, Mayer S (1978) Influence of the major histocompatibility complex in the pig (SLA) on serum haeolytic complement levels. J Immunogenet 5:59–65
Vaiman M, Chardon P, Renard Ch (1979) Genetic organization of the pig SLA complex. Studies of nine recombinant and biochemical and lysostrip analysis. Immunogenetics 9:353–361
Vaiman M, Renard Ch, Bourgeaux N (1988) The SLA complex, the major histocompatibility system in swine: its influence on physiological and pathological traits. In: Warner CM, Rothschild MF, Lamont SJ (eds) Proceedings of International Symposium on the Molecular Biology of the Major Histocompatibility Complex of Domestic Animal Species Iowa Sate University Press, Ames, Iowa, pp 23–38
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Communicated by L. D. Van Vleck
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Jung, Y.C., Rothschild, M.F., Flanagan, M.P. et al. Genetic variability between two breeds based on restriction fragment length polymorphisms (RFLPs) of major histocompatibility complex class I genes in the pig. Theoret. Appl. Genetics 77, 271–274 (1989). https://doi.org/10.1007/BF00266197
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DOI: https://doi.org/10.1007/BF00266197