Summary
We have transplanted cultured cells derived from a human giant cell tumour of bone (G-1 cell) into immunologically suppressed mice. The resulting growths were morphologically and cytokinetically analyzed. We have obtained information on the cytokinetic influences of anti-cancer agents on heterotransplanted tumours using the double labelling method.
Tumour formation was noted in 56 out of 76 mice, i.e. 76%. In 3 mice which had been kept under observation the large tumours led to death.
The heterotransplanted tumours of G-1 cells had morphologically malignant and non-epithelial characteristics. The cells appeared to have undergone malignant change during their long period of cultivation.
Using the double labelling method, 5-FU was found to prolong the DNA synthetic time and decrease the cell birth rate of G-1 cells in vivo. Accordingly, 5-FU is the most effective anti-cancer agent to G-1 cells.
In order to improve the prognosis in a malignant bone tumour the most effective anti-cancer agent should be determined by testing on a heterotransplanted tumour derived from the patient.
Résumé
Les auteurs ont greffé des cellules cultivées à partir d'une tumeur osseuse à cellules géantes humaine (cellule G-1) sur des souris ayant subi une suppression immunitaire. Les tumeurs résultantes ont été analysées sur le plan morphologique et cytocinétique. Grâce à la technique du double marquage, on a obtenu des informations concernant les influences cytocinétiques des agents anticancéreux sur les tumeurs hétérotransplantées.
Des tumeurs se sont développées chez 56 souris sur 76, soit 76%. Chez trois animaux soumis à une observation prolongée, on a constaté que les volumineuses tumeurs qui se sont formées ont entraîné la mort.
Les tumeurs hétérotransplantées des cellules G-1 étaient morphologiquement malignes et non épithéliales. Les cellules ont apparemment subi une transformation maligne pendant leur longue période de culture.
A l'aide du double marquage on a trouvé que le 5-fluoro-uracile prolongeait le temps de synthèse de l'ADN tandis qu'il diminuait le taux de division des cellules G-1 in vitro. Le 5-fluoro-uracile est donc l'agent anticancéreux le plus efficace à l'égard des cellules G-1.
Pour améliorer le pronostic d'une tumeur osseuse maligne, on devrait déterminer l'agent anticancéreux le plus efficace en le testant sur une tumeur hétérotransplantée dérivée du malade.
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Manabe, S., Takase, B., Yamazaki, Y. et al. Heterotransplantation of the cultured cell from a human giant cell tumour of bone. International Orthopaedics 2, 69–76 (1978). https://doi.org/10.1007/BF00266005
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DOI: https://doi.org/10.1007/BF00266005