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Cell culture amplification of a defective Marek's disease virus

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Abstract

A highly amplified 4-kb EcoRI fragment was present in DNA isolated from high cell culture passaged stocks (>93 passages) of 281MI/1, a serotype 2 Marek's disease virus (MDV). The isolated 4-kb fragment is amplified in the presence of MDV, replicating as a high molecular weight, head-to-tail concatemer. When the 4-kb fragment was cloned into pUC18 and cotransfected with MDV DNA into chicken embryo fibroblast cells, the plasmid clone also replicated as a high molecular weight concatemer.

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Author notes

  1. Robert F. Silva

    Present address: Agricultural Research Service, Regional Poultry Research Laboratory, U.S. Department of Agriculture, East Lansing, MI, USA

Authors and Affiliations

  1. Agricultural Research Service, Regional Poultry Research Laboratory, U.S. Department of Agriculture, East Lansing, MI, USA

    Jeanne K. Carter

Authors
  1. Jeanne K. Carter
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  2. Robert F. Silva
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Carter, J.K., Silva, R.F. Cell culture amplification of a defective Marek's disease virus. Virus Genes 4, 225–237 (1990). https://doi.org/10.1007/BF00265632

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  • DOI: https://doi.org/10.1007/BF00265632

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