Summary
The tif-1 mutation has been shown to affect protein synthesis in vitro by increasing translational ambiguity (Ephrati-Elizur, Luther-Davies and Hayes, 1976). It is demonstrated here that some recA mutations confer similar abnormality. By comparing suitable combinations of ribosomes and soluble proteins from recA + and recA cells the defect is shown to be associated with ribosomes. The recA mutation, which suppresses most phenotypic characteristics of the tif-1 mutation (Castellazzi, George and Buttin, 1972(b)) does not suppress the ribosomal abnormality. Sience the closely linked tif-1 and recA mutations lead to the expression of a common property they may be in the same gene.
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Communicated by B.A. Bridges
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Powell, K.A., Emmerson, P.T. Ribosomal abnormality in recA mutants of Escherichia coli . Molec. Gen. Genet. 154, 83–86 (1977). https://doi.org/10.1007/BF00265580
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DOI: https://doi.org/10.1007/BF00265580