Molecular and General Genetics MGG

, Volume 154, Issue 1, pp 61–66 | Cite as

Characterization of a regulatory mutant of fructose 1,6-bisphosphatase in Saccharomyces carlsbergensis

  • K. W. van de Poll
  • D. H. J. Schamhart


The fdp mutation has been localized on the genome of Saccharomyces carlsbergensis, on chromosome II, between lys2 and tyr1, at a map distance of 31 centimorgan from lys2.

Since the fdp mutant does not grow on glucose, fructose, mannose and sucrose, hexose transport and a number of enzymes of carbon metabolism were tested, but no significant differences could be found between the wild type and the mutant. Only the regulatory properties of glycogen synthetase are changed in the mutant, but it is doubtfull whether this can explain its phenotype.

The disorganization of carbon metabolism of the mutant upon addition of glucose to the medium was analyzed in more detail. The most prominent feature observed until now is the accumulation of free glucose and hexose phosphates in the cell. This result indicates that somehow the feedback control between hexose transport and metabolism is impaired. Hexose phosphates are known to be toxic to many cells, including yeast. Therefore, accumulation of hexose phosphates in the presence of glucose in the medium, can explain the absence of growth on this carbon source.


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  1. Azam, F., Kotyk, A.: Glucose-6-phosphate as regulator of monosaccharide transport in baker's yeast. Fed. Eur. Biochem. Soc. Lett. 2, 333–335 (1969)Google Scholar
  2. Bergmeyer, H.U.: Methods of enzymatic analysis. New York and London: Academic Press, Inc. 1974Google Scholar
  3. Cirillo, V.P.: Galactose transport in Saccharomyces cerevisiae. J. Bact. 95, 1727–1731 (1968)Google Scholar
  4. Gancedo, C.: Inactivation of fructose-1,6-diphosphatase by glucose in yeast. J. Bact. 107, 401–405 (1971)Google Scholar
  5. Gancedo, M.M., Lagunas, R.: Contribution of the pentose-phosphate pathway to glucose metabolism in Saccharomyces cerevisiae: a critical analysis on the use of labelled glucose. Plant Sci. Letters 1, 193–200 (1973)Google Scholar
  6. Herrera, L.S., Pascual, C., Alvarez, X.: Genetic and biochemical studies of phosphomannose isomerase deficient mutants of Saccharomyces cerevisiae. Molec. gen. Genet. 144, 223–230 (1976)Google Scholar
  7. Jaspers, H.T.A., Van Steveninck, J.: Transport associated phosphorylation of 2-deoxy-d-glucose in Saccharomyces fragilis. Biochim. biophys. Acta (Amst.) 406, 370–385 (1975)Google Scholar
  8. Kotyk, A., Michaljaničová, D.: Nature of the uptake of d-galactose, d-glucose and α-methyl-d-glucoside by Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 332, 104–113 (1974)Google Scholar
  9. De Kroon, R.A., Koningsberger, V.V.: An inducible transport system for α-glucosides in protoplasts of Saccharomyces carlsbergensis. Biochim. biophys. Acta (Amst.) 204, 590–609 (1970)Google Scholar
  10. Kuo, S.-C., Lampen, J.O.: Inhibition by 2-deoxy-d-glucose of synthesis of glycoprotein enzymes by protoplasts of Saccharomyces: relation to inhibition of sugar uptake and metabolism. J. Bact. 111, 419–429 (1972)Google Scholar
  11. Lampen, J.O.: External enzymes of yeast: their nature and formation. Antonie van Leeuwenhoek. J. Microbiol. Serol. 34, 1–18 (1968)Google Scholar
  12. Maitra, P.K.: Glucose and fructose metabolism in a phosphoglucoisomeraseless mutant of Saccharomyces cerevisiae. J. Bact. 107, 759–769 (1971)Google Scholar
  13. Mortimer, R.K., Hawthorne, D.C.: Yeast genetics; In: The yeasts (Rose, A.H., Harrison, J.S., eds.), Vol. I, pp, 385–460. New York and London: Academic Press, Inc. 1969Google Scholar
  14. Mortimer, R.K., Hawthorne, D.C.: Genetic mapping in Saccharomyces IV. Genetics 74, 33–54 (1973)Google Scholar
  15. Pringle, J.R.: Genetic and physiological characterization of a mutation which prevents glycogen accumulation in yeast. Genetics 71, S48 (1972)Google Scholar
  16. Rothman, L.B., Cabib, E.: Regulation of glycogen synthesis in the intact yeast cell. Biochemistry 8, 3332–3341 (1969)Google Scholar
  17. Rothman-Denes, L.B., Cabib, E.: Two forms of yeast glycogen synthetase and their role in glycogen accumulation. Proc. nat. Acad. Sci. (Wash.) 66, 967–974 (1970)Google Scholar
  18. Sols, A.: Regulation of carbohydrate transport and metabolism in yeast, In: Aspects of yeast metabolism (A.K. Mills, ed.), pp. 47–70. Oxford and Edinburgh: Blackwell Scientific Publications 1966Google Scholar
  19. Ten Berge, A.M.A.: Genes for the fermentation of maltose and α-methylglucoside in Saccharomyces carlsbergensis. Molec. gen. genet. 115, 80–88 (1972)Google Scholar
  20. Van de Poll, K.W.: Repression and derepression of allantoinase synthesis in protoplasts of Saccharomyces carlsbergensis. Proc. kon. ned. Akad. Wet. B 73, 342–356 (1970)Google Scholar
  21. Van de Poll, K.W., Kerkenaar, A., Schamhart D.H.J.: Isolation of a regulatory mutant of fructose-1,6-diphosphatase in Saccharomyces carlsbergensis. J. Bact. 117, 965–970 (1974)Google Scholar
  22. Van Steveninck, J.: Transport and transport-associated phosphorylation of 2-deoxy-d-glucose in yeast. Biochim. biophys. Acta (Amst.) 163, 386–394 (1968)Google Scholar
  23. Van Steveninck, J.: Transport and transport-associated phosphorylation of galactose in Saccharomyces cerevisiae. Biochim. biophys. Acta (Amst.) 274, 575–583 (1972)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • K. W. van de Poll
    • 1
  • D. H. J. Schamhart
    • 1
  1. 1.Department of Molecular Cell Biology, Section of Molecular BiologyState University of UtrechtUtrechtThe Netherlands

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