Summary
Electrophoretic comparisons of sucrose synthetase (SS) proteins in complementing heterozygotes and the corresponding in vitro mixtures of extracts from the homozygotes are described. The latter revealed two protein bands in the expected fashion. The SS protein pattern in the hybrid was different from that of the mixtures. The possibility of heteromeric SS molecules, formed by random polymerization of subunits of the tetramer coded by each allele in the heterozygote, was considered. Such an interaction was expected to form a multiple of five SS proteins that could be visualized after gel electrophoresis. However, only two SS bands were seen in the hybrids. The basis of this marked deviation remains to be explained.
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Literature
Beckman, L.; Scandalios, J.G.; Brewbaker, J. (1964): Catalase hybrid enzymes in maize. Science 146, 1174–75
Chourey, P.S. (1971): Interallelic complementation at the Sh 1 locus in maize. Genetics 68, 434–442
Chourey, P.S.; Schwartz, D. (1971): Ethyl methanesulfonate induced mutations at the Sh 1 protein in maize. Mutat. Res. 12, 151–157
Chourey, P.S.; Nelson, O.E. (1976): The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem. Genet. 14, 1041–1055
Chourey, P.S.; Nelson, O.E. (1979): Interallelic complementation at the sh locus in maize at the enzyme level. Genetics 91, 317–325
Coddington, A.; Fincham, J.R.S. (1965): Proof of hybrid protein formation in a case of interallelic complementation in Neurospora crassa. J. Mol. Biol. 12, 152–161
Crick, F.H.C.; Orgel, L.E. (1964): The theory of interallelic complementation. J. Mol. Biol. 8, 161–165
Davis, B.J. (1964): Disc electrophoresis, II. Method and applicacation to human serum protein. Ann. N.Y. Acad. Sci. 121, 404–427
Delmer, D.P. (1972): The purification and properties of sucrose synthetase from etiolated Phaseolus aureus seedlings. J. Biol. Chem. 247, 3822–3828
Doring, H.P.; Ehring, R.; Geiser, M.; Starlinger, P.; Wostemeyer, J. (1980): The gene encoding endosperm sucrose synthetase in Zea mays as a tool to study controlling elements. In: Fourth John Innes Symp. and Second Int'l. Haploid Conf. (eds.: Davies, D.R.; Hopwood, D.A.) pp. 73–77. Norwich: John Innes Institute.
Finnerty, V.; Johnson, G. (1979): Post-translational modification as a potential explanation of high levels of enzyme polymorphism: Xanthine dehydrogenase and aldehyde oxidase in Drosophila melanogaster Genetics 91, 695–722
Judd, B.H. (1979): Allelic complementation and transveotion in Drosophila melanogaster. In: ICN-UCLA Symp. Molec. and Cellular Biol XIV, 107–115
Kida, S.; Crawford, I.P. (1974): Complementation in vitro between mutationally altered B2 subunits of E. coli tryptophan synthetase. J. Bacteriol. 118, 551–559
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J. (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275
Nomura, T.; Akazawa, T. (1973): Enzymatic mechanism of starch synthesis in ripening rice grains. VII. Purification and enzymatic properties of sucrose synthetase. Arch. Biochem. Biophys. 156, 644–652
Schlesinger, M.J.; Levinthal, C. (1963): Hybrid protein formation of E. coli alkaline phosphatase leading to in vitro complementation. J. Mol. Biol. 7, 1–12
Schwartz, D. (1960): Electrophoretic and immunochemical studies with endosperm proteins of maize mutants. Genetics 45, 1419–1427
Schwartz, D. (1971): Genetic control of alcohol dehydrogenase — a competition model for regulation of gene action. Genetics 67, 411–425
Schwartz, D. (1975): The molecular basis for allelic complementation of alcohol dehydrogenase mutants of maize. Genetics 79, 207–212
Su, J.C.; Preiss, J. (1978): Purification and properties of sucrose synthetase from maize kernels. Plant Physiol. 61, 389–393
Weber, K.; Osborn, M. (1969): The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406–4412
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Communicated by: O.E. Nelson, Jr.
Cooperative Investigation, United States Department of Agriculture and Institute of Food and Agricultural Sciences, University of Florida, Florida Agricultural Experiment Station Journal Series No. 2470.
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Chourey, P.S. Electrophoretic analysis of sucrose synthetase proteins in the complementing heterozygotes at the Shrunken locus in maize. Theoret. Appl. Genetics 59, 231–234 (1981). https://doi.org/10.1007/BF00265500
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DOI: https://doi.org/10.1007/BF00265500