Summary
Three annual widespread species of Hordeum were investigated by the fragment pattern method on their chloroplast (cp) DNA. The species were H. glaucum, H. leporinum and H. murinum; H. vulgare was surveyed for comparison. Twelve restriction enzymes were used, nine recognizing 6 bp, one 5 bp and two 4 bp, thus, randomly surveyed, a total of 2,113 bp or 1.6% of the cp genome. Differences in patterns were found in three enzymes, HindIII, CfoI and MspI. CfoI characterizes H. glaucum from the other two species. HindIII and MspI revealed polymorphisms within species. These results confirm previous numerical taxonomic relationships among these three closely related species. Furthermore, cpDNA polymorphism in Hordeum is discussed in view of earlier reports on cpDNA polymorphism in H. vulgare. The taxonomic implications of cpDNA polymorphism are discussed after reviewing several articles using the fragment pattern method on cpDNA. The importance of using material from several populations representative of a species is stressed.
Similar content being viewed by others
References
Baatout H, Marakchi M, Vedel F (1985) Variation of plastid and mitochondrial DNAs in the genus Hedysarum. Theor Appl Genet 70:577–584
Banks JA, Birky Jr CW (1985) Chloroplast DNA diversity is low in a wild plant, Lupinus texensis. Proc Natl Acad Sci USA 82:6950–6954
Baum BR, Bailey LG (1984 a) Taxonomic studies in wall barley (Hordeum murinum) and sea barley (Hordeum marinum). 1. Character investigation: assessment of new and traditional characters. Can J Bot 62:753–762
Baum BR, Bailey LG (1984 b) Taxonomic studies in wall barley (Hordeum murinum sensu lato) and sea barley (Hordeum marinum senu lato). 2. Multivariate morphometrics. Can J Bot 62:2754–2764
Baum BR, Bothmer R von, Jacobsen N, Fedak G, Craig I, Bailey LG (1984) Barley gene pool; a collection maintained by Agriculture Canada and by the Danish and Swedish agricultural universities (Nordic Gene Bank); Canadian-Scandinavian Hordeum Collection (CHC). Publication 1761/B, Communications Branch, Agriculture Canada, Ottawa
Berthou F, Mathieu C, Vedel F (1983) Chloroplast and mitochondrial DNA variation as indicator of phylogenetic relationships in the genus Coffea L. Theor Appl Genet 65:77–84
Buckner B, Hyde BB (1985) Chloroplast DNA variation between the common cultivated potato (Solanum tuberosum ssp. tuberosum) and several South American relatives. Theor Appl Genet 71:527–531
Clegg MT, Brown AHD, Whitfeld PR (1984 a) Chloroplast DNA diversity in wild and cultivated barley: implications for genetic conservation. Genet Res 43:339–343
Clegg MT, Rawson JRY, Thomas K (1984 b) Chloroplast DNA variation in Pearl millet and related species. Genetics 106:449–461
Coates D, Cullis CA (1987) Chloroplast DNA variability among Linum species. Am J Bot 74:260–268
Cockerill PN (1988) Two methods that facilitate autoradiography of small 32P-labeled DNA fragments following electrophoresis in agarose gels. Anal Biochem 168:451–454
Enomoto S-I, Ogihara Y, Tsunewaki K (1985) Studies on the origin of crop species by restriction endonuclease analysis of organellar DNA. I. Phylogenetic relationships among ten cereals revealed by the restriction fragment patterns of chloroplast DNA. Jpn J Genet 60:411–424
Fitch WM (1984) Cladistic and other methods: problems, pitfalls, and potentials. In: Duncan T, Stuessy TF (eds) Claditics: perspectives on the reconstruction of evolutionary history. Columbia University Press, New York, pp 221–252
Holwerda BC, Jana S, Crosby WL (1986) Chloroplast DNA and mitochondrial DNA variation in Hordeum vulgare and Hordeum spontaneum. Genetics 114:1271–1291
Hosaka K (1986) Who is the mother of the potato? — Restriction endonuclease analysis of chloroplast DNA of cultivated potatoes. Theor Appl Genet 72:606–618
Jansen RK, Palmer JD (1988) Phylogenetic implications of chloroplast DNA restriction site variation in the Mutisieae (Asteraceae). Am J Bot 75:753–766
Kataoka J, Ogihara Y, Tsunewaki K (1987) Chloroplast DNA variation observed among Hordeum species. Barley Genet 5:515–524
Kung SD, Zhu YS, Shen GF (1982) Nicotiana chloroplast genome. 3. Chloroplast DNA evolution. Theor Appl Genet 61:73–79
Lehväslaiho H, Saura A, Lokki J (1987) Chloroplast DNA variation in the grass tribe Festuceae. Theor Appl Genet 74:298–302
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Milo J, Levy A, Ladizinsky G, Palevitch D (1988) Phylogenetic and genetic studies in Papaver section Oxytona: cytogenetics, isozyme analysis and chloroplast DNA variation. Theor Appl Genet 75:795–802
Ogihara Y, Tsunewaki K (1982) Molecular basis of the genetic diversity of the cytoplasm in Triticum and Aegilops. I. Diversity of the chloroplast genome and its lineage revealed by the restriction patterns of ctDNAs. Jpn J Genet 57:371–396
Palmer JD (1987) Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130:S6-S29
Palmer JD, Zamir D (1982) Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proc Natl Acad Sci USA 79:5006–5010
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Poulsen C (1983) The barley chloroplast genome: pyhsical structure and transcriptional activity in vivo. Carlsberg Res Commun 48:57–80
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer, Dordrecht, pp A6:1–10
Snow R (1963) Alcoholic hydrochloric acid carmine as a stain for chromosomes in squash preparations. Stain Technol 38:9–13
Swofford DL, Berlocher SH (1987) Inferring evolutionary trees from gene frequency data under the principle of maximum parsimony. Syst Zool 36:293–325
Sytsma KJ, Gottlieb LD (1986 a) Chloroplast DNA evidence for the origin of the genus Heterogaura from a species of Clarkia (Onagraceae). Proc Natl Acad Sci USA 83:5554–5557
Sytsma KJ, Gottlieb LD (1986 b) Chloroplast DNA evolution and phylogenetic relationships in Clarkia Sect. Perpetasma (Onagraceae). Evolution 40:1248–1261
Sytsma KJ, Schaal BA (1985) Phylogenetics of the Lisianthius skinneri (Gentianaceae) species complex in Panama utilizing DNA restriction fragment analysis. Evolution 39:594–608
Timothy DH, Levings III CS, Pring DR, Conde MF, Kermicle JL (1979) Organelle DNA variation and systematic relationships in the genus Zea: Teosinte. Proc Natl Acad Sci USA 76:4220–4224
Vedel F, Lebacq P (1980) Cytoplasmic DNA variation and relationships in cereal genomes. Theor Appl Genet 58:219–224
Vedel F, Quetier F, Dosba F, Dousinnault G (1978) Study of wheat phylogeny by EcoRI analysis of chloroplast and mitochondrial DNAs. Plant Sci Lett 13:97–102
Yanagino T, Takahata Y, Hinata K (1987) Chloroplast DNA variation among diploid species in Brassica and allied genera. Jpn J Genet 62:119–125
Zurawski G, Clegg MT (1987) Evolution of higher-plant chloroplast DNA-encoded genes: implications for structure-function and phylogentic studies. Annu Rev Plant Physiol 38:391–418
Author information
Authors and Affiliations
Additional information
Communicated by K. Tsunewaki
Rights and permissions
About this article
Cite this article
Baum, B.R., Bailey, L.G. Species relationships in the Hordeum murinum aggregate viewed from chloroplast DNA restriction fragment patterns. Theoret. Appl. Genetics 78, 311–317 (1989). https://doi.org/10.1007/BF00265289
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00265289