Skip to main content
Log in

Magnification of the rDNA cluster in Kluyveromyces lactis

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

By employing pulsed field gel electrophoresis we find that slow growing strains of Kluyveromyces lactis have only 43%–55% of the wild-type level of ribosomal DNA (rDNA) repeats. When subjected to prolonged vegetative growth these strains can increase both the number of rDNA repeats and their growth rate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bianchi MM, Falcone C, Jie CX, Wesolowski-Louvel M, Frontali L, Fukuhara H (1987) Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 gm plasmid pKD1. Curr Genet 12:185–192

    CAS  Google Scholar 

  • Garber RC, Turgeon BG, Selker EU, Yoder OC (1988) Organization of ribosomal RNA genes in the fungus Cochhobolus heterostrophus. Curr Genet 14:573–582

    Google Scholar 

  • Maleszka R, Clark-Walker GD (1989) A petite positive strain of Kluyveromyces lactis has a 300kb deletion in the rDNA cluster. Curr Genet 16:429–432

    Google Scholar 

  • Russell PJ, Rodland KD (1986) Magnification of rRNA gene number in a Neurospora crassa strain with a partial deletion of the nucleolus organizer. Chromosoma 93:337–340

    Google Scholar 

  • Schwartz DC, Cantor CR (1984) Separation of yeast chromosomesized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75

    Google Scholar 

  • Sollner-Webb B, Tower J (1986) Transcription of cloned eukaryotic ribosomal RNA genes. Annu Rev Biochem 55:801–830

    Google Scholar 

  • Szostak JW, Wu R (1980) Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature 284:426–430

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by C.P. Hollenberg

Rights and permissions

Reprints and permissions

About this article

Cite this article

Maleszka, R., Clark-Walker, G.D. Magnification of the rDNA cluster in Kluyveromyces lactis . Molec. Gen. Genet. 223, 342–344 (1990). https://doi.org/10.1007/BF00265074

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00265074

Key words

Navigation