Summary
The general outline of meiotic prophase proposed by Wilson in 1925 included three stages which usually are missing in the description of meiosis today: (1) synizesis (= the synaptic stage in plants), (2) the diffuse stage, which follows the pachytene and (3) the second contraction, following the diffuse stage. The last two stages were regarded by Wilson as being very variable and could even be lacking. These stages, described by early plant cytologists later became regarded as fixation artefacts and after the introduction of the squash technique were either misinterpreted and/or forgotten.
However, all three stages are easily recognised in aceticethanol fixed preparations of plant meiosis and are demonstrated in Pisum sativum meiosis. Pisum meiosis differs from the usual plant meiosis by: (1) the peculiar timing of the late prophase, (2) the existence of extrachromosomal fibrillar structures appearing at synizesis and staining by Feulgen — Light Green, and (3) the appearance of multiple nucleoli in the late prophase. The possibility that these nucleoli and fibrillar structures are of relevance in relation to gene amplification is discussed.
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Literature
Atabekova, A.J. (1959): Cytologische Untersuchungen an der Kulturerbse Pisum sativum. Biol. Zbl. 3, 424–438
Bier, K.; Kunz, W.; Ribbert, D. (1967): Struktur und Funktion der Oocytenchromosomen und Nukleolen sowie der Extra-DNS während der Oogenese panoistischer und meroistischer Insekten. Chromosoma 23, 214–254
Cannon, W.A. (1903): Studies in plant hybrids: the spermatogenesis of hybrid peas. Bull. Torrey Bot. Club 30, 510–543
Cooper, G. (1938): Cytological investigations of Pisum sativum. Bot. Gaz. 99, 584–591
Gall, J.G. (1968): Differential synthesis of the genes for ribosomal RNA during amphibian oogenesis. Proc. Nat. Acad. Sci. (Wash.) 60, 553–560
Gall, J.G. (1969): The genes for ribosomal RNA during oogenesis. Genetics (suppl.) 61, 121–132
Gottschalk, W.; Baquar, S.R. (1971): Desynapsis in Pisum sativum induced through gene mutation. Can. J. Genet. Cytol. 13, 138–143
Gottschalk, W.; Baquar, S.R. (1973): Highly unstable translocations induced through irradiation in Pisum. Cytologia 38, 327–336
Gottschalk, W.; Milutinović, M. (1970): Die Analyse hochgradiger Chromosomenverkettungen in der Meiosis von Pisum sativum. Caryologia 23, 473–488
Gottschalk, W.; Villabos-Petrini, R. (1965): The influence of mutant genes on chiasma formation. Cytologia 30, 88–97
Håkansson, A. (1931): Über Chromosomenverkettung in Pisum. Hereditas 15, 17–61
Håkansson, A.; Levan, A. (1942): Nucleolar conditions in Pisum. Hereditas 28, 436–440
Klášterská, I. (1977): The concept of the prophase of meiosis. Hereditas 86, 205–210
Klášterská, I. (1978): Structure of eukaryotic chromosomes: The differences between mammalian (mouse), grasshopper (Stethophyma) and plant (Rosa) chromosomes as revealed at the diffuse stage of meiosis. Hereditas 88, 243–253
Klášterská, I.; Natarajan, A.T. (1974): Cytological studies of the genus Rosa with special reference to the section Caninae. Hereditas 76, 97–108
Klášterská, I.; Ramel, C. (1979): Prophase of plant meiosis: sequences and interpretation of stages. Genetica 51, 15–20
Klášterská, I.; Ramel, C. (1980a): Sequence and interpretation of meiotic prophase stages in PMCs of Najas marina (Najadaceae). Hereditas 92, 171–175
Klášterská, I.; Ramel, C. (1980b): Meiosis in PMCs of Arabidopsis thaliana. Arabidopsis Inf. Serv. 17, 1–10
Klein, H.D. (1971): Eine Pisum Mutante mit zahlreichen meiotischen Störungen. Cytologia 36, 15–25
Klein, H.D. (1972): Genetic control of chromosome morphology. Pisum Newsl. 4, 12–13
Lamm, R. (1976): Technical hints for chromosome studies in Pisum. Hereditas 84, 235–237
Lewitsky, G.A. (1927): Die Bildung bivalenter Chromosomen in der Gonogenese von Beta vulgaris L. Planta 3, 100–114
Morrison, J.W.; Lin, S.C. (1955): Chromosomes and nucleoli in Pisum sativum. Nature 175, 343–344
Sharp, L.W. (1926): An Introduction to Cytology. 2. ed. New York: McGraw-Hill
Williams, E.; Heslop-Harrison, J.; Dickinson, H.G. (1973): The activity of the nucleolus organising region and the origin of cytoplasmic nucleoloids in meiocytes of Lilium. Protoplasma 77, 79–93
Wilson, E.B. (1925): The Cell in Development and Heredity. 3. edition. New York: McMillan
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Communicated by Å. Gustafsson
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Klášterská, I., Ramel, C. Peculiarities in PMC Meiosis of Pisum sativum . Theoret. Appl. Genetics 59, 297–302 (1981). https://doi.org/10.1007/BF00264981
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DOI: https://doi.org/10.1007/BF00264981