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An evaluation of mitochondrial heterosis and in vitro mitochondrial complementation in wheat, barley and maize

Theoretical and Applied Genetics volume 59, pages 153–160 (1981)Cite this article

Summary

Two families each of wheat (Triticum aestivum L.), barley (Hordeum vulgare L.) and maize (Zea mays L.) were studied for mitochondrial heterosis and in vitro mitochondrial complementation. Inbred parents and their hybrids were compared for seedling heights and rate of oxygen uptake by the whole tissue to find out if the hybrids showed greater growth and respiratory activity at the seedling stage. Further comparisons were made by isolating mitochondria from the seedling tissues and measuring their ADP∶0 ratio, respiratory control ratio and cytochrome c oxidase activity for mitochondrial heterosis. Mixtures of parental mitochondria were similarly compared with parental and hybrid mitochondria for in vitro mitochondrial complementation. No evidence for mitochondrial heterosis or in vitro mitochondrial complementation was found, nor any correlation between the different mitochondrial parameters, seedling heights or rates of oxygen uptake by seedling tissue. The suggested use of mitochondrial heterosis and in vitro mitochondrial complementation for plant breeding is discussed.

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Affiliations

  1. Department of Soil & Crop Sciences, Texas A & M University, College Station, Texas, USA

    D. Sen

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  1. D. Sen
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Additional information

Data for this paper is taken from the author's dissertation written as a part of Ph.D. degree requirements at the Biology Department, Texas A & M University, College Station, Texas

Communicated by O.E. Nelson

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Sen, D. An evaluation of mitochondrial heterosis and in vitro mitochondrial complementation in wheat, barley and maize. Theoret. Appl. Genetics 59, 153–160 (1981). https://doi.org/10.1007/BF00264969

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  • DOI: https://doi.org/10.1007/BF00264969

Key words

  • Mitochondrial heterosis
  • Mitochondrial complementation
  • Triticum aestivum
  • Hordeum vulgare
  • Zea mays