In chickpea, out of three colchicine concentrations and two treatment durations used (combinations of 0.25, 0.05, 0.025% colchicine and 4 and 6 h duration), seed treatment with 0.25% for 4 h proved to be the most effective in producing autotetraploids. Colchicine treatment on seedlings failed. The induced tetraploidy was accompanied by larger leaves, flowers, stomata, pollen grains and seeds. Mean percentage stainable pollen and podset were reduced, but some plants had relatively normal meiosis and produced as many pods as the diploid parent, indicating the potential of induced autotetraploids in chickpea improvement.
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Approved as J. A. No. 265 by the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
Communicated by G. S. Khush
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Pundir, R.P.S., Rao, N.K. & van der Maesen, L.J.G. Induced autotetraploidy in chickpea (Cicer arietinum L.). Theoret. Appl. Genetics 65, 119–122 (1983). https://doi.org/10.1007/BF00264878