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A selection procedure for obtaining conditional gametogenic mutants using a photosynthetically incompetent strain of Chlamydomonas reinhardi

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Summary

A new selection procedure has been developed for the isolation of temperature sensitive gametogenic mutants in Chlamydomonas reinhardi. This technique makes use of pet-10-1, a mutant strain incapable of photosynthetic electron transport, and the redox dye, methyl viologen. Two mutants found by this method are discussed. The first, gam-4, does not agglutinate at 35°, whereas the second, gam-5, is capable of sexual agglutination, but not of zygote formation at the restrictive temperature. Both mutants appear to be inherited in a Mendelian fashion and are expressed in mating type (+) and mating type (-).

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Author notes

  1. Charlene L. Forest

    Present address: The Biological Laboratories, Harvard University, 16 Divinity Avenue, 02138, Cambridge, Mass, U.S.A.

Authors and Affiliations

  1. Department of Zoology, Department of Plant Sciences, Indiana University, 47401, Bloomington, Ind, U.S.A.

    Charlene L. Forest & Robert K. Togasaki

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  1. Charlene L. Forest
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  2. Robert K. Togasaki
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Communicated by W. Gilbert

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Forest, C.L., Togasaki, R.K. A selection procedure for obtaining conditional gametogenic mutants using a photosynthetically incompetent strain of Chlamydomonas reinhardi . Molec. Gen. Genet. 153, 227–230 (1977). https://doi.org/10.1007/BF00264740

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  • DOI: https://doi.org/10.1007/BF00264740

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