Summary
Two cytoplasmic, glucosamine resistant (GR) mutants of Saccharomyces cerevisiae, GR6 and GR10, were crossed to strains bearing known mitochondrial markers. Analysis of vegetative and meiotic segregation patterns in these crosses suggested that the glucosamine resistance conferring factor did not reside on mitDNA. This was confirmed by ethidium bromide treatments which completely abolished oligomycin resistance due to a mitochondrial mutation at the OLI2 locus but which failed to eliminate the GR factor present in the same strain. Comparison of GR6 and GR10 to some other known cytoplasmic determinants suggested that while glucosamine resistance is not related to the killer plasmid it may be allelic to the URE3 determinant and/or to the PSI factor.
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Aigle, M., Lacroute, F.: Genetical aspects of [URE3], a non-mitochondrial, cytoplasmically inherited mutation in yeast. Molec. gen. Genet. 136, 327–335 (1975)
Avner, P.R., Coen, D., Dujon, B., Slonimski, P.P.: Mitochondrial Genetics. IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae. Molec. gen. Genet. 125, 9–52 (1973)
Ball, A.J.S., Wong, D.K., Elliot, J.J.: Glucosamine resistance in yeast. I. A preliminary genetic analysis. Genetics 84, 311–317 (1976)
Birky, C.W., Jr.: On the origin of mitochondrial mutants: evidence for intracellular selection of mitochondria in the origin of antibiotic-resistant cells in yeast. Genetics 72, 421–432 (1974)
Bussey, H.: Effects of yeast killer factor on sensitive cells. Nature (Lond.) New Biol. 235, 73–75 (1972)
Cox, B.: ψ a cytoplasmic suppressor of super-suppressor in yeast. Heredity 20, 505–521 (1965)
Dujon, B., Slonimski, P.P., Weill, L.: Mitochondrial genetics. IX. A model for recombination and segregation of mitochondrial genomes in Saccharomyces cerevisiae. Genetics 78, 415–437 (1974)
Elliot, J.J., Ball, A.J.S.: Glucosamine resistance in the yeast Saccharomyces cerevisiae. Genetics, 74, S71 (1973)
Elliot, J.J., Ball, A.J.S.: A preliminary analysis of glucosamine resistance in yeast. Proc. Canad. Fed. Biol. Soc. 17, 50 (1974)
Elliot, J.J., Ball, A.J.S.: A new mitochondrial mutation in yeast. Biochem. biophys. Res. Commun. 64, 277–281 (1975)
Fink, G.R., Styles, C.A.: Curing of a killer factor in Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 69, 2846–2849
Griffiths, D.E., Lancashire, W.E., Zanders, E.D.: Evidence for an extrachromosomal element involved in mitochondrial function: a mitochondrial episome? FEBS Lett. 53, 126–130 (1975)
Grivell, L.A., Netter, P., Borst, P., Slonimski, P.P.: Mitochondrial antibiotic resistance in yeast. Ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin. Biochim. biophys. Acta (Amst.) 312, 358–367 (1973)
Guerineau, M., Slonimski, P.P., Avner, P.R.: Yeast episome: oligomycin resistance associated with a small covalently closed nonmitochondrial circular DNA. Biochem. biophys. Res. Commun. 61, 462–469 (1974)
Ibsen, K.H.: The Crabtree effect: A review. Cancer Res. 21, 829–841 (1961)
Lacroute, F.: Non Mendelian mutation allowing ureidosuccinic acid uptake in yeast. J. Bact. 106, 519–522 (1971)
Makower, M., Bevan, E.A.: The inheritance of a killer character in yeast (S. Cerevisiae). Proc. Int. Congr. Genet. XI 1, 202 (1963)
Netter, P., Petrochilo, E., Slonimski, P.P., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Dujon, B.: Mitochondrial genetics VII. Allelism and mapping studies of ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin in S. cerevisiae. Genetics 78, 1063–1100 (1974)
Perlman, P.S., Demko, C.A.: Effects of gene dosage on mitochondrial marker transmission in Saccharomyces cerevisiae. Genetics 77, S50 (Abst.) (1974)
Racker, E.: History of the Pasteur effect and its pathobiology. Molec. Cellular Biochem. 5, 17–23 (1974)
Rank, G.H., Bech-Hansen, N.T.: Somatic segregation, recombination, asymmetrical distribution and complementation tests of cytoplasmically-inherited antibiotic resistance mitochondrial markers in S. cerevisiae. Genetics 72, 1–15 (1972)
Saunders, G.W., Gingold, E.B., Trembath, M.K., Lukins, H.B., Linnane, A.W.: Mitochondrial genetics in yeast. Segregation of a cytoplasmic determinant in crosses and its loss or retention in the petite. In: Autonomy and biogenesis of mitochondria and chloroplasts (Boardman, N.K., Linnane, A.W., Smillie, R.M., eds.), pp. 185–193. New York: North Holland 1971
Sherman, F., Fink, G., Lukins H.B.: Laboratory manual for a course: Methods in yeast genetics. Cold Spring Harbour, New York: Cold Spring Harbour Laboratory for Quantitative Biology 1970
Somers, J.M.: Isolation of suppressive sensitive mutants from killer and neutral strains of Saccharomyces cerevisiae. Genetics 74, 571–579 (1973)
Thomas, D.Y., Wilkie, D.: Recombination of mitochondrial drug resistance in Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 30, 368–372 (1968)
Vodkin, M.H., Katterman, F., Fink, G.R.: Yeast killer mutants with altered double-stranded ribonucleic acid. J. Bact. 117, 681–686 (1974)
Wickner, R.B.: Chromosomal and non-chromosomal mutations affecting “killer character” of S. cerevisiae: Curing by growth at elevated temperatures. J. Bact. 117, 1356–1357 (1974b)
Wolf, K., Dujon, B., Slonimski, P.P.: Mitochondrial Genetics V. Multifactorial mitochondrial crosses involving a mutation conferring paronomycin resistance in Sacharomyces cerevisiae. Molec. gen. Genet. 125, 53–90 (1973)
Young, C.S.H., Cox, B.: Extrachromosomal elements in super suppression system of yeast III. Enhanced detection of weak suppressors in certain non-suppressed PSI strains. Heredity 34, 83–92 (1975)
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Communicated by F. Kaudewitz
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Kunz, B.A., Ball, A.J.S. Glucosamine resistance in yeast. Molec. Gen. Genet. 153, 169–177 (1977). https://doi.org/10.1007/BF00264732
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DOI: https://doi.org/10.1007/BF00264732