Abstract
A streaming fluid containing a bleachable fluorescent tracer is illuminated by an intense laser beam having a steep intensity profile and a wavelength suitable to induce either the fluorescence or the bleaching reaction. Providing the beam intensity is sufficiently high to bleach all the dye molecules crossing the beam, the fluorescence intensity is proportional to the rate of transport of the dye into the illuminated region. This phenomenon makes it possible to measure out two-dimensional cross-sections of complex velocity fields with microscopic spatial resolution and within fractions of a second. The measurable velocity range is limited only by the diffusion of the dye on its lower end and by the bleaching sensitivity and the available laser power on the upper end. The applicability of this principle is illustrated in the example of Bénard-Marangoni flow.
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Rička, J. Photobleaching velocimetry. Experiments in Fluids 5, 381–384 (1987). https://doi.org/10.1007/BF00264401
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DOI: https://doi.org/10.1007/BF00264401