Summary
The study of phenolic content and activities of peroxidase and polyphenoloxidase in relation to the degree of downy mildew infection of 12 pearl millet cultivars revealed that these were linearly related to the degree of resistance at both the 30 and 50 day growth stages. Useful electrophoretic differences in peroxidase and polyphenoloxidase were also observed with respect to the expression of resistance.
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Bateman DF (1967) Increase in polyphenoloxidase in diseased plant tissue. In: Source book of laboratory excercises in plant pathology. Am Phytopathol Soc Freeman, San Francisco, pp 208–209
Gupta VK, Stebbins GL (1969) Peroxidase activity in hooded and awned barley at successive stages of development. Biochem Genet 3:5–24
Lowry OH, Rosenbrough NJ, Farr A, Randall RJ (1951) Protein measurement with the folinphenol reagent. J Biol Chem 193:265–275
Murty BR (1980) Developments and lacunae in the genetic improvement of Pennisetums. In: Gupta VP, Minocha JL (eds) Trends in genetical research on Pennisetums. Punjab Agricultural University, Ludhiana, pp 9–18
Shannon L, Key E, Lew J (1966) Peroxidase isozymes from horseradish roots. 1. Isolation and physical properties. J Biol Chem 241:2166–2175
Swain T, Hillis WC (1959) The phenolic constituents of Prunus domestica (I). The quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68
Thakur DP, Kanwar JS (1977) Infectivity of sporangia of Sclerospora graminicola in pearl millet downy mildew. Indian J Mycol Plant Pathol 7:104–105
Vanloon LC (1971) Tobacco polyphenoloxidases: A specific staining method indicating non-identity with peroxidase. Phytochemistry 10:503–507
Veech JA (1969) Localization of peroxidase in infected tobaccos, susceptible and resistant to black shank. Phytopathology 59:560–571
Williams RJ, Singh SD, Thakur RP (1976) Rating scales and standard drawings for incidence and severity assessment of pearl millet diseases. 1. Downy mildew, ergot and smut. ICRISAT, Hyderabad, India
Williams RJ, Singh SD, Pawar MN (1981) An improved field screening technique for downy mildew resistance in pearl millet. Plant Dis 65:239–241
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Communicated by J. MacKey
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Thukral, S.K., Satija, D.R. & Gupta, V.P. Biochemical genetic basis of downy mildew resistance in pearl millet. Theoret. Appl. Genetics 71, 648–651 (1986). https://doi.org/10.1007/BF00264270
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DOI: https://doi.org/10.1007/BF00264270