Summary
Protoplasts of 6-azauracil (AU) resistant cell lines of Solanum melongena L. were fused with protoplasts of S. sisymbriifolium Lam. to create somatic hybrids between these sexually-incompatible species. Following fusion, colonies were selected which were capable of growth in medium containing 1mM AU. These colonies were placed on medium containing zeatin which had been shown to stimulate anthocyanin production during shoot organogenesis in tissue explants of S. sisymbriifolium but not in S. melongena. A total of 37 anthocyanin-producing colonies were identified from which 26 hybrid plants were regenerated. The morphological traits intermediate to those of the parents included: flower colour, leaf shape, and trichome density. Cytogenetic analysis revealed that all hybrids were aneuploids but their chromosome numbers were close to the expected number of 48. Isozyme analysis revealed that nuclear genes of both parents were expressed in the hybrids. In addition, isoelectric focussing of the large subunit of ribulose 1,5-bisphosphate carboxylase (Rubisco) provided evidence that each hybrid expressed only the S. sisymbriifolium chloroplast genome. All hybrids regenerated thus far have been sterile.
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References
Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Somatic hybridization in the genus Solanum; S. tuberosum and S. brevidens. Plant Cell Rep 3:165–167
Binding H, Jain SM, Finger J, Mordhorst G, Nehls R, Gressel J (1982) Somatic hybridization of an atrazine-resistant biotype of Solanum nigrum with Solanum tuberosum. 1. Clonal variation in morphology and in atrazine sensitivity. Theor Appl Genet 63:273–277
Butenko R, Kuchko A, Komarnitsky I (1982) Some features of somatic hybrids between Solanum tuberosum and S. chacoense and its F1 sexual progeny. In: Fujiwara F (ed) Plant tissue culture 1982. Jpn Ass Plant Tissue Culture, Tokyo, pp 643–644
Douglas GC, Keller WA, Setterfield G (1981 a) Somatic hybridization between Nicotiana rustica and N. tabacum. 2. Protoplast fusion and selection and regeneration of hybrid plants. Can J Bot 59:1509–1513
Douglas GC, Wetter LR, Nakamura C, Keller WA, Setterfield G (1981 b) Somatic hybridization between Nicotiana rustica and N. tabacum. 3. Biochemical morphological, cytological analysis of somatic hybrids. Can J Bot 59:228–237
Dudits D, Fejer O, Hadlaczy G, Koncz C, Lazar G, Horvath C (1980) Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet 179:283–288
Evans DA, Wetter LR, Gamborg OL (1980) Somatic hybrid plants of Nicotiana glauca + Nicotiana tabacum obtained by protoplast fusion. Physiol Plant 48:225–2530
Evans DA, Flick CE, Kut SA, Reed SM (1982) Comparison of Nicotiana tabacum and Nicotiana nesophila hybrids produced by ovule culture and protoplast fusion. Theor Appl Genet 62:193–198
Fassuliotis G (1975) Regeneration of whole plants from isolated stem parenchyma cells of Solanum sisymbriifolium. J Am Soc Hortic Sci 100:636–638
Fassuliotis G, Bhatt DP (1982) Potential of tissue culture for breeding root-knot nematode resistance into vegetables. J Nematol 14:10–14
Gleddie S, Fassuliotis G, Keller WA, Setterfield G (1985 a) Somatic hybridization as a potential method of transferring nematode and mite resistance into eggplant. Z Pflanzenzücht 94:348–351
Gleddie S, Keller WA, Setterfield G (1983 a) Somatic embryogenesis and plant regeneration from leaf explants and cell suspensions of Solanum melongena (eggplant). Can J Bot 61:656–666
Gleddie S, Keller WA, Setterfield G, Wetter LR (1983 b) Somatic hybridization between Nicotiana rustica and N. sylvestris. Plant Cell Tissue Organ Cult 2:269–283
Gleddie S, Keller WA, Setterfield G (1985 b) Somatic embryogenesis and plant regeneration from cell suspension derived protoplasts of Solanum melongena L. Can J Bot (in press)
Gleddie S, Keller WA, Setterfield G (1985 c) Plant regeneration from tissue cell, and protoplast cultures of several wild Solanum species. J Plant Physiol 119:405–418
Iwai S, Nakata K, Nagao T, Kawashima N, Matsuyama S (1980) Detection of the Nicotiana rustica chloroplast genome coding for the large subunit of Fraction 1 protein in a somatic hybrid in which only the N. tabacum chloroplast genome appeared to have been expressed. Planta 152:478–480
Kao KN (1977) Chromosomal behavior in somatic hybrids of soybean-Nicotiana glauca. Mol Gen Genet 150:225–230
Lowe RH (1977) Crystallization of Fraction 1 protein from tobacco by a simplified procedure. FEBS Lett 78:98–100
Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43:203–218
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Power JB, Berry SF, Chapman JV, Cocking EC (1980) Somatic hybridization of sexually incompatible petunias: Petunia parodii, Petunia parviflora. Theor Appl Genet 57:1–4
Schalk JM, Stoner AK, Webb RE, Winters HF (1975) Resistance in eggplant, Solanum melongena L. and nontuber-bearing Solanum species to carmine spider mite. J Am Soc Hortic Sci 100:479–481
Schieder O (1978) Somatic hybridization of Datura innoxia (Mill) and Datura discolor (Bern L) and of Datura innoxia (Mill)+Datura stramonium L. var tatula. 1. Selection and characterization. Mol Gen Genet 162:113–119
Schieder O (1982) In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York, pp 239–253
Scowcroft WR, Larkin PJ (1981) Chloroplast DNA assorts randomly in intraspecific somatic hybrids of Nicotiana debneyi. Theor Appl Genet 60:179–184
Seck A (1983) Amelioration génétique de l'aubergine: étude des crossments de Solanum melongena L. par S. torvum et S. sisymbriifolium. Memoire de Premiére Année (Thesis) Montpellier
Shepard JF, Bidney D, Barsby T, Kemble R (1983) Genetic transfer in plants through interspecific protoplast fusion. Science 219:683–688
Sidorov JF, Menczel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts. Planta 152:341–345
Smillie RM, Melchers G, Von Wettstein D (1979) Chilling resistance of somatic hybrids of tomato and potato. Carlsberg Res Commun 44:127–132
Snow R (1963) Alcoholic hydrochloric acid — carmine as a stain for chromosomes in squash preparations. Stain Technol 38:9–13
Wetter LR (1977) Isoenzyme patterns in soybean-Nicotiana somatic hybrid cell lines. Mol Gen Genet 150:231–235
Wetter L, Dyck J (1983) Isoenzyme analysis of cultured cells and somatic hybrids. In: Evans D, Sharp W, Ammirato P, Yamada Y (eds) Handbook of plant cell culture, vol 1. MacMillan, New York, pp 607–628
Wetter LR, Kao KN (1980) Chromosome and isoenzyme studies on cells derived from protoplast fusion of Nicotiana glauca with Glycine max-Nicotiana glauca. Theor Appl Genet 57:273–276
Wright WE (1978) The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors. Exp Cell Res 112:395–407
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Communicated by R. Riley
Contribution No. 787 Ottawa Research Station
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Gleddie, S., Keller, W.A. & Setterfield, G. Production and characterization of somatic hybrids between Solanum melongena L. and S. sisymbriifolium Lam.. Theoret. Appl. Genetics 71, 613–621 (1986). https://doi.org/10.1007/BF00264265
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DOI: https://doi.org/10.1007/BF00264265