Summary
A R 401 Waters Assoc. differential refractometer has been modified to obtain the variation of refractive index increments, dn/dc, of polymer solutions at 633 nm. The introduced modifications were in the sample injection system and in the light source. The performance of the modified refractometer both with aqueous and organic solutions is analyzed. In order to localize a conformational transition in lysozyme, the dependence of dn/dc on temperature for lysozyme solutions in phosphate buffer is studied. Similarly, (dn/dc)k values for the ternary n-alkane/butanone/poly(dimethyl siloxane) systems over the whole composition range of the binary solvent mixtures are evaluated.
Similar content being viewed by others
References
ABAD, C., TRUEBA, M. ZATON, A. and CAMPOS, A.: An. Quim. 75, 993(1979)
BODMAN, O.: Makromol. Chem. 122, 196(1969)
CAMPOS, A., CELDA, B. TEJERO, R. and FIGUERUELO, J.E.: Europ. Polym. J. 20, 447(1984)
CAMPOS, A., CELDA, B., MORA, J. and FIGUERUELO, J.E.: Polymer (in press a)
CAMPOS, A., CELDA, B., MORA, J. and FIGUERUELO, J.E.: Europ. Polym. J. (in press b)
CANTOW, H.-J. and BODMAN, O.: Z. Phys. Chem. 3, 65(1955)
CELDA, B.: PhD These, Valencia University, Valencia (Spain)
COZZONE, P., OFELLA, J.J., JARDIZKY, O., BERTHOV, J. and JOLLES, P.: Proc. Natl. Acad. Sci. US 72, 2095(1975)
ELIAS, H.G. and ETTER, O.: Makromol. Chem. 66, 56(1963)
ESKIN, V.E., IZYUMNIKOV, A.L., ROGOZHKINA, E.D. and VYRSKII, Yu.P.: Polym. Sci. USSR 7, 1310(1965)
HUGLIN, M.B.: Light Scattering from Polymer Solutions, London: Academic Press 1972
HUGLIN, M.B.: Polymer Handbook, 2nd Ed., New York: Wiley-Interscience 1975
IMS: Instruction Manual Series R 400, Waters Assoc. Inc. Mass. USA 1976
JOLLES, P.: Febbs Lett. 55, 165(1975)
KATIME, I. and ROIG, A.: An. Quim. 70, 853(1974)
KRUIS, A.Z.: Physik. Chem. B 34, 13(1936)
MILLAUD, B. and STRAZIELLE, C.: Makromol. Chem. 180, 441(1979)
RIDDIK, J.A. and BUNGGER, W.B.: Organic Solvents, Vol. 2, 3th Ed., New York: Interscience Publis. Inc. 1970
SAIN BLANCHARD, J., CLOCHARD, A., COZZONE, P.J. and JOLLES, P.: Biochim. Biophys. Acta 491, 354(1977)
SILBERBERG, A., ELIASSAF, J. and KATCHALSKY, A.: J. Polym. Sci. 23, 259(1957)
SORIA, V., CAMPOS, A. and FIGUERUELO, J.E.: An. Quim. 74, 1026(1978)
SYNOVEC, R.E. and YEUNG, E.S.: Anal. Chem. 55, 1599(1983)
WIGAND, G. and WEITH, J.J.: Past. und Kaut. 16, 671(1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Soria, V., Llopis, A., Celda, B. et al. Static measurements of refractive index increments at 633 nm with a modified refractive index detector. Polymer Bulletin 13, 83–88 (1985). https://doi.org/10.1007/BF00264245
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00264245