Summary
Two genomic clones, pC1.2 and p20D (containing inserts of 2.0 and 1.6 kb, respectively) were isolated from the A2b region to polytene chromosome IV of Chironomus thummi thummi salivary gland cells. Upon in situ hybridization to polytene chromosomes of C. thummi thummi and C. thummi piger, p20D DNA hybridized mainly over the A2b region of chromosome IV, whereas pC1.2 DNA hybridized to at least 90 sites distributed over all the chromosomes. A partial nucleotide sequence analysis showed that these clones were very similar and allowed the detection of a 596 by insert in the pC1.2 clone. This insert possesses all of the essential features of a Class II transposable element and was called MEC. It carries a nearly perfect 107 by terminal inverted repeat containing one mismatch and is flanked by a 5 by direct repeat. The 372 by central region contains a short open reading frame with a coding capacity of 58 amino acids.
References
Finnegan DJ (1989) Eucaryotic transposable elements and genome evolution. Trends Genet 5:103–107
Finnegan DJ, Fawcett DH (1986) Transposable elements in Drosophila melanogaster. In: Maclean N (ed) Oxford Surveys on Eucaryotic Genes. Oxford University Press, Oxford, pp 1–63
Hägele K (1970) DNS-Replikationsmuster der Speicheldrüsen-Chromosomen von Chironomidan. Chromosoma 31:91–138
Kiknadze H, Kolesnikov NN, Panova TM, Gaydamakova EK, Blinov AG, Filippova MA (1987) Transposable elements in Chironomidae (in Russian). Genetika 23:1366–1376
Rubin GM (1983) Dispersed Repetitive DNAs in Drosophila. In: Shapiro (ed) Mobile Genetic Elements. Academic Press, N.Y., pp 329–361
Schmidt ER, Godwin EA, Keyl HG, Israelevski N (1982) Cloning and analysis of ribosomal DNA of Chironomus thummi thummi and Chironomus thummi piger. Chromosoma 87:389–407
Tchuvpilo SA, Krawchenko WW (1983) A solid-phase method for DNA sequencing (in Russian). Bioorg Khim (Moskow) 9:1634–1637
Wobus U, Baumlein H, Bogachev SS, Borisevich IW, Panitz R, Kolesnikov NN (1990) A new transposable element in Chironomus thummi. Mol Gen Genet 222:311–316
Author information
Authors and Affiliations
Additional information
Communicated by J. Finnegan
Rights and permissions
About this article
Cite this article
Blinov, A.G., Sobanov, Y.V., Gaidamakova, E.K. et al. MEC: A transposable element from Chironomus thummi (Diptera). Molec. Gen. Genet. 229, 152–154 (1991). https://doi.org/10.1007/BF00264224
Issue Date:
DOI: https://doi.org/10.1007/BF00264224