Abstract
Complementation tests and enzyme analyses separated 29 leucine auxotrophs of the Basidiomycete Rhodosporidium toruloides into three groups, each deficient in one of the leucine biosynthetic enzymes. The following differences are suggested between the organization of the leucine pathway in R. toruloides and the Ascomycetes Saccharomyces cerevisiae and Neurospora crassa: (1) isopropylmalate, the product of the first enzymic reaction appears not to be an internal inducer of the later enzymes of the pathway; this is consistent with the apparent lack of mutants homologous to the leu3 class in N. crassa and S. cerevisiae; (2) as in S. cerevisiae, but unlike N. crassa, isopropylmalate synthase is under the control of a general cross pathway control system; (3) unlike S. cerevisiae, but like N. crassa, R. toruloides appears to possess only one gene encoding isopropylmalate synthase.
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Abbreviations
- IPM :
-
Isopropylmalate
- EMS :
-
methanesulphonic acid, ethyl ester
References
Baichwal VR, Cunningham TS, Gatzek PR, Kohlhaw GB (1983) Leucine biosynthesis in yeast: identification of two genes (LEU4, LEU5) that affect isopropylmalate synthase activity and evidence that LEU1 and LEU2 gene expression is controlled by isopropylmalate and the product of a regulatory gene. Curr Genet 7:369–377
Banno I (1967) Studies on the sexuality of Rhodotorula. J Gen Appl Microbiol 13:176–196
Bigelis R (1981) The yeast leu-1 gene encodes isopropylmalate isomerase. Microb Genetics Bull 50:11–12
Bode R, Casper P (1983) Allgemeine Kontrolle der Aminosäurebiosynthese-Mutanten von Candida sp. EH15/D. Z Allg Mikrobiol 23:419–427
Bottcher F, Samsonova IA (1978) Rhodosporidium Banno: Dosiseffekt-beziehungen, Mutageneffektivität und Mutantenspektrum bei der Induktion Auxotrophie verursachender Mutationen durch Ultra-voilettes Licht and N-methyl N′-nitro-N-nitrosoguanidin. Z Allg Mikrobiol 18:637–646
Bottcher F, Samsonova IA (1980) Genetische Konsequenzen sexueller Kreuzungen bei Rhodosporidium toruloides. Wiss Z Ernst Moritz Arndt Univ 29:39–40
Carsiotis M, Jones RF, Wesseling AC (1974) Cross pathway regulation: histidine mediated control of histidine, tryptophan and arginine biosynthetic pathways in Neurospora crassa. J Bacteriol 119:838–898
Ciriacy M (1975) The genetics of alcohol dehydrogenase in Saccharomyces cerevisiae: I. isolation and genetic analysis of adh mutants. Mutat Res 29:315–326
Delforge J, Messenguy F, Wiame JM (1975) The regulation of arginine biosynthesis in Saccharomyces cerevisiae: the specificity of argR mutations and the general control of amino acid biosynthesis. Eur J Biochem 57:231–239
Fantes PA, Roberts LM, Hutter R (1976) Free tryptophan pool and tryptophan biosynthetic enzymes in Saccharomyces cerevisiae. Arch Microbiol 107:207–214
Flint HJ, Kemp BF (1981) General control of arginine biosynthetic enzymes in Neurospora crassa. J Gen Microbiol 124:129–140
Gilbert HJ, Tully M (1982) Synthesis and degradation of phenylalanine ammonia-lyase of Rhodosporidium toruloides. J Bacteriol 150:498–505
Gross SR (1965) The regulation of synthesis of the leucine biosynthetic enzymes of Neurospora. Proc Natl Acad Sci USA 54: 1538–1546
Gross SR (1971) Isopropylmalate isomerase (Neurospora). Methods Enzymol 17B:766–790
Henry SA, Donahue TF, Culbertson MR (1982) Selection of spontaneous mutants by inositol starvation in yeast. Mol Gen Genet 143:5–11
Hinnebusch AG (1992) General and pathway-specific regulatory mechanisms controlling the synthesis of amino acid biosynthetic enzymes in Saccharomyces cerevisiae. In: Jones EW, Pringle JR, Broach JR (eds) The molecular and cellular biology of the yeast Saccharomyces, vol 2. Gene expression. Cold Spring Harbor Press, Cold Spring Harbor, NY, pp 319–390
Hsu Y-P, Kohlhaw GB (1980) Leucine biosynthesis in Saccharomyces cerevisiae: purification and characterization of β-isopropylmalate dehydrogenase. J Biol Chem 255:7255–7260
Hsu Y-P, Schimmel P (1984) Yeast LEU1: repression of mRNA levels by leucine and relationship of 5′-noncoding region to that of LEU2. J Biol Chem 259:3714–3719
Hsu Y-P, Kohlhaw GB, Niederberger P (1982) Evidence that isopropylmalate synthase of Saccharomyces cerevisiae is under the ‘general’ control of amino acid biosynthesis. J Bacteriol 150:969–972
Jones EW, Fink GR (1982) Regulation of amino acid and nucleotide biosynthesis in yeast. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces, vol 2. Metabolism and gene expression. Cold Spring Harbor Press, Cold Spring Harbor, NY, pp 181–299
Kinzel JJ, Bhattacharjee JK (1979) Role of pipecolic acid in the biosynthesis of lysine in Rhodotorula glutinis. J Bacteriol 138: 410–417
Kirsop BE (1984) Maintenance of yeasts. In: Kirsop BE, Snell JJS (ed) Maintenance of micro-organisms: a manual of laboratory methods. Academic Press, New York London, pp 109–130
Kohlhaw GB (1982) Regulation of leucine biosynthesis in lower eukaryotes. In Hermann HR, Somerville RS (eds) Biotechnology: amino acid biosynthesis and regulation, vol 1. Addison-Wesley, Reading, Mass, pp 285–299
Kohlhaw GB, Leary TR (1971) Isopropylmalate synthase (Salmonella typhimurium). Methods Enzymol 17B:771–773
Kusaka I, Fukui S (1975) Morphological change in the early stages of the mating process of Rhodosporidium toruloides. J Bacteriol 122:710–718
Miozzari GF, Niederberger P, Hutter R (1978) Permeabilization of micro-organisms by Triton X-100. Anal Biochem 90:220–233
Noltmann EA, Gubler CJ, Kuby SA (1961) Glucose 6-phosphate dehydrogenase (Zwischenferment) I: Isolation of the crystalline enzyme from yeast. J Biol Chem 236:1225–1230
Piotrowska M (1980) Cross pathway regulation of ornithine carbamoyl-transferase synthesis in Aspergillus nidulans. J Gen Microbiol 116:335–339
Polacco JC, Gross SR (1973) The product of the leu3 cistron as a regulatory element for the production of the leucine biosynthetic enzymes of Neurospora. Genetics 74:443–459
Reichenbecher YE, Fischer M, Gross SR (1979) Regulation of isopropylmalate synthesis in Neurospora crassa. J Bacteriol 133: 794–801
Sherman F, Fink GR, Hicks JB (1986) Laboratory course manual for methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Soda K (1967) A spectrophotometric microdetermination of keto acids with 3-methyl-2-benzothiazolone hydrazone. Agric Biol Chem 31:1054–1060
Tamir H, Srinivasan PR (1970) Anthranilate synthetase (Salmonella typhimurium). Methods Enzymol 17A:401–406
Tully M (1985) Enrichment of mutants of Rhodosporidium toruloides by the use of inositol starvation. J Basic Microbiol 25: 683–686
Tully M, Gilbert HI (1985) Transformation of Rhodosporidium toruloides. Gene 36:235–240
Wade HE, Phillips BP (1971) Automated determination of bacterial asparaginase and glutaminase. Anal Biochem 44:189–199
Wolfe RG, Nielands JB (1956) Some molecular and kinetic properties of heart malic dehydrogenase. J Biol Chem 221:61–69
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Tully, M. Leucine biosynthesis and its regulation in the basidiomycete Rhodosporidium toruloides . Arch. Microbiol. 162, 362–367 (1994). https://doi.org/10.1007/BF00263785
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DOI: https://doi.org/10.1007/BF00263785