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Phase separation of polymerized lipids in hybrid liposomes

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Summary

Hybrid liposomes were prepared from the mixture of dipalmitoyl phosphatidylcholine (DPPC) and di-2,4-octadecadiene phosphatidylcholine (DODPC), and their phase separation behaviours was analyzed. In hybrid liposome systems, two transition temperatures (18 and 41°C for DODPC and DPPC, respectively) were clearly observed by means of DSC and fluorescence techniques. These two transition temperatures were also found after polymerization of DODPC molecules in hybrid liposomes by the UV irradiation. These strongly suggest that DPPC and DODPC molecules do not dissolve well but form clusters in the membrane. This phase separation was directly confirmed by freeze-fracture TEM technique.

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Authors and Affiliations

  1. Department of Polymer Chemistry, Waseda University, 160, Tokyo, Japan

    Noriko Seki, Eishun Tsuchida & Kazuo Ukaji

  2. Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi 40, 500, Gifu, Japan

    Takashi Sekiya & Yoshinori Nozawa

Authors
  1. Noriko Seki
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  2. Eishun Tsuchida
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  3. Kazuo Ukaji
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  4. Takashi Sekiya
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  5. Yoshinori Nozawa
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Seki, N., Tsuchida, E., Ukaji, K. et al. Phase separation of polymerized lipids in hybrid liposomes. Polymer Bulletin 13, 489–492 (1985). https://doi.org/10.1007/BF00263468

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  • DOI: https://doi.org/10.1007/BF00263468

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