Summary
A new method of enzyme immobilization by a polyion complex was proposed. An enzyme immobilizing membrane was prepared by ultrafiltrating a mixture consisted of quarternized chitosan, sodium polyacrylate and invertase in an aqueous NaBr solution. The permeation and hydrolysis characteristics of aqueous sucrose solution through the invertase immobilizing membrane was studied under some conditions. A hydrolysis rate of sucrose by the invertase immobilizing membrane corresponded to the Michael is-Menten type reaction.
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References
Uragami, T. Fujino, K. and Sugihara, M.: Angew. Makromol. Chem. 55, 29 (1976)
Uragami, T. Tamura, M. and Sugihara, M.: Angew. Makromol, Chem. 55, 59 (1976); 66, 203 (1978); J. Membr. Sci. 4, 305 (1979)
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Uragami, T., Aketa, T., Gobodani, S. et al. Studies of syntheses and permeabilities of special polymer membranes. Polymer Bulletin 15, 101–106 (1986). https://doi.org/10.1007/BF00263385
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DOI: https://doi.org/10.1007/BF00263385