Summary
In rats given cycloheximide to prevent ACTH from stimulating corticosterone biosynthesis, the lipid droplets in the zona fasciculata were found to have turned polymorphic, grown larger, and increased in number. In the absence of eorticosterone production 3H-cholesterol accumulated in the lipid droplets. The digitonin reaction was used to identify free cholesterol for electron microscopy.
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The authors are indebted to Dr. Gy. Rappay and L. Csáki for helpful advice and to Miss É. Kovács for expert assistance.
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Szabó, D., Dzsinich, C. & Ökrös, I. Ultrastructural localisation of adrenal cholesterol by autoradiography and digitonin reaction after cycloheximide-induced inhibition of corticosterone synthesis. Histochemie 27, 43–49 (1971). https://doi.org/10.1007/BF00263367
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DOI: https://doi.org/10.1007/BF00263367