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Theoretical and Applied Genetics

, Volume 58, Issue 3–4, pp 97–100 | Cite as

The nucleolus organizers of diploid wheats revealed by in situ hybridization

  • W. L. Gerlach
  • T. E. Miller
  • R. B. Flavell
Article

Summary

Labelled RNA, transcribed in vitro from wheat ribosomal DNA cloned in a bacterial plasmid, has been hybridised to metaphase chromosomes of five diploid wheats. Autoradiography of the chromosomes has provided unequivocal evidence that these genotypes possess two pairs of nucleolus organizer chromosomes. The diploid wheat accessions used possess widely differing numbers of ribosomal RNA genes.

Key words

Nucleolus organizers In situ hybridisation Diploid wheats Ribosomal RNA genes 

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Literature

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • W. L. Gerlach
    • 1
  • T. E. Miller
    • 1
  • R. B. Flavell
    • 1
  1. 1.Department of CytogenticsPlant Breeding InstituteTrumpingtonEngland

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