Abstract
A new monoxenic culture of the anaerobic ciliate Trimyema compressum was established on the purple nonsulfur bacterium Rubrivivax gelatinosus. Compared with the first monoxenic culture of T. compressum on a Bacteroides species no significant difference in growth rate was observed. Using both monoxenic cultures the fermentation products of the ciliate, acetate, lactate, formate and succinate were quantified. Ammonium was another product of the ciliates metabolism. The amount of the endproducts was dependent on the amount of bacteria consumed. The ratio of carbon consumed to ammonium formed was 8.7:1 indicating incomplete degradation of nitrogenous compounds of the bacterial biomass. Under microoxic conditions no effect of oxygen on the yield of the ciliate was observed but the amount of acetate and lactate was 25% lower. Cells of T. compressum with Methanobacterium formicicum as deliberately introduced endosymbiont did not form significantly different fermentation products, however, instead of formate methane was detected as product. The yield of the ciliate was not affected. It is concluded that methanogenic bacteria deliberately introduced into the cytoplasm of T. compressum as endosymbionts are pure commensalists.
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References
Bauchop T, Mountfort DO (1981) Cellulose fermentation by a rumen anaerobic fungus in both the absence and the presence of rumen methanogens. Appl Environ Microbiol 42: 1103–1110
Bruggen JJA van, Stumm CK, Vogels GD (1983) Symbiosis of methanogenic bacteria and sapropelic protozoa. Arch Microbiol 136: 89–95
Bruggen JJA van, Zwart KB, Assema RM van, Stumm CK, Vogels GD (1984) Methanobacterium formicicum, an endosymbiont of the anaerobic ciliate Metopus striatus McMurrich. Arch Microbiol 139: 1–7
Bruggen JJA van, Stumm CK, Zwart KB, Vogels GD (1985) Endosymbiotic methanogenic bacteria of the sapropelic amoeba Mastigella. FEMS Microbiol Ecol 31: 187–192
Bruggen JJA van, Zwart KB, Hermans JGF, Hove EM van, Stumm CK, Vogels GD (1986) Isolation and characterization of Methanoplanus endosymbiosus sp. nov., an endosymbiont of the marine sapropelic ciliate Metopus contortus. Arch Microbiol 144: 367–374
Bruggen JJA van, Rens GLM van, Geertman EJM, Zwart KB, Stumm CK, Vogels GD (1988) Isolation of a methanogenic symbiont of the sapropelic amoeba Pelomyxa palustris Greef. J Protozool 35: 20–23
Coleman GS (1967) The metabolism of the amino acids of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum. J Gen Microbiol 47: 449–464
Coleman GS, Hall FJ (1972) Fine structural studies on digestion of bacterial species in the rumen ciliate, Entodinium caudatum. Tissue Cell 4: 37–48
DEV (1983) Deutsche Einheitsverfahren zur Wasser-, Abwasser-und Schlamm-Untersuchung. Eds. Fachgruppe Wasserchemie in der Gesellschaft Deutscher Chemiker in Gemeinschaft mit dem Normenausschuß Wasserwesen (NAW) im DIN Deutsches Institut für Normung e.V., vol 2, section E5. Verlag Chemie, Weinheim, FRG
Doddema HJ, Vogels GD (1978) Improved identification of methanogenic bacteria by fluorescence microscopy. Appl Environ Microbiol 36: 752–754
Ellis JE, Williams AG, Lloyd D (1989) Oxygen consumption by ruminal microorganisms: protozoal and bacterial contributions. Appl Environ Microbiol 55: 2583–2587
Goosen NK, Horemans AMC, Hillebrand SJW, Stumm CK, Vogels GD (1988) Cultivation of the sapropelic ciliate Plagiopyla nasuta Stein and isolation of the endosymbiont Methanobacterium formicicum. Arch Microbiol 150: 165–170
Goosen NK, Wagener S, Stumm CK (1990a) A comparison of two strains of the anaerobic ciliate Trimyema compressum. Arch Microbiol 153: 187–192
Goosen NK, Drift C van der, Stumm CK, Vogels CD (1990b) End products of metabolism in the anaerobic ciliate Trimyema compressum. FEMS Microbiol Lett 69: 171–176
Guttierez J (1955) Experiments on the culture and physiology of holotrichs from the bovine rumen. Biochem J 60: 516–522
Howard BH (1959) The biochemistry of rumen protozoa. 1. Carbohydrate fermentation by Dasytricha and Isotricha. Biochem J 71: 671–675
Lindmark DG, Müller M (1973) Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Tritrichomonas foetus and its role in pyruvate metabolism. J Biol Chem 248: 7724–7728
Lindmark DG, Eckenrode BL, Halberg LA, Dinbergs ID (1989) Carbohydrate, energy and hydrogenosomal metabolism of Tritrichomonas foetus and Trichomonas vaginalis. J Protozool 36: 214–216
Mack SR, Müller M (1980) End products of carbohydrate metabolism in Trichomonas vaginalis. Comp Biochem Physiol 67B: 213–216
McLaughlin J, Alex S (1985) The biochemistry and functional morphology of the Entamoeba. J Protozool 32: 221–240
Montalvo FE, Reeves RE, Warren G (1971) Aerobic and anaerobic metabolism in Entamoeba histolytica. Exp Parasitol 30: 249–256
Müller M (1988) Energy metabolism of protozoa without mitochondria. Ann Rev Microbiol 42: 465–488
Pfennig N (1978) Rhodocyclus purpureus gen. nov. and spec. nov., a ring-shaped vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28: 283–288
Reeves RE (1984) Metabolism of Entamoeba histolytica Schaudinn, 1903. Adv Parasitol 23: 105–142
Ryley JF (1955) Studies on the metabolism of the protozoa: 5. metabolism of the parasitic flagellate Trichomonas foetus. Biochem J 59: 361–369
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Holler, S., Pfennig, N. Fermentation products of the anaerobic ciliate Trimyema compressum in monoxenic cultures. Arch. Microbiol. 156, 327–334 (1991). https://doi.org/10.1007/BF00263006
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DOI: https://doi.org/10.1007/BF00263006