Urological Research

, Volume 17, Issue 5, pp 299–303 | Cite as

Analysis of mucosal bladder leucocyte subpopulations in patients treated with intravesical Bacillus Calmette-Guerin

  • M. Peuchmaur
  • G. Benoit
  • A. Vieillefond
  • A. Chevalier
  • G. Lemaigre
  • E. D. Martin
  • A. Jardin
Original Articles

Summary

Immunohistochemical techniques were used to investigate leucocyte subpopulations in the bladders of patients with superficial transitional cell carcinoma treated with BCG Pasteur. Leucocyte subsets were enumerated with a panel of monoclonal antibodies which included CD3, CD4, CD8, TQ1, Leu7, CD15, HLA-DR, CD25, CD22. We demonstrated in the bladders of patients treated with BCG a particular lymphocyte population; the major subset was an inducer (CD4+, TQ1-) which was activated (CD25+, HLA-DR+) and associated with polymorphonuclear eosinophils. There was neither inducer of suppression nor major cytotoxic/suppressive subsets. CD8+ and NK cells could not be the primary mediators of BCG activity. These data supported the hypothesis of a helper T lymphocyte activity associated with lymphokine production and activation of effector killer cells.

Key words

Carcinoma of the bladder BCG therapy Immunohistochemical studies Leucocyte subpopulations Helper T lymphocyte 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Peuchmaur
    • 1
  • G. Benoit
    • 2
  • A. Vieillefond
    • 3
  • A. Chevalier
    • 1
  • G. Lemaigre
    • 1
  • E. D. Martin
    • 3
  • A. Jardin
    • 2
  1. 1.Department of PathologyAntoine Béclère HospitalClamart
  2. 2.Department of UrologyBicêtre HospitalLe Kremlin BicêtreFrance
  3. 3.Department of PathologyBicêtre HospitalLe Kremlin BicêtreFrance

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