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Haemocyanin oxygen binding and the physiological ecology of a range of talitroidean amphipods (Crustacea) II. Effect of freezing, inorganic ions, and urate on O2 binding in vitro

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Summary

The effect of variations in [K], [Ca], [Mg], [NaCl], and [urate] on the in vitro O2 binding properties of haemocyanin (Hc) from three talitroidean species, viz. the aquatic Apohyale pugettensis, the semi-terrestrial Megalorchestia californiana, and the semi-/euterrestrial Traskorchestia traskiana were studied. Freezing altered the cooperativity of Hc from A. pugettensis and M. californiana but not T. traskiana. Variations in [NaCl], [K], and [Mg] had no effect on cither O2 affinity or cooperativity of the Hc except for A. pugettensis Hc where an increase in [Mg] resulted in an increase in both O2 affinity and cooperativity. Increasing [Ca] or [urate] increased O2 affinity of both A. pugettensis and M. californiana but not T. traskiana Hc. These effects were most marked in A. pugettensis. The results suggest a negative correlation between sensitivity to Hc effectors and the degree of terrestrial adaptation of a particular amphipod species.

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Abbreviations

Hc:

haemocyanin

STR:

Stock traskorchestia ringer

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Author notes

  1. John I. Spicer

    Present address: Department of Zoology, University of Glasgow, G12 8QQ, Glasgow, UK

Authors and Affiliations

  1. Department of Biological Sciences, University of Calgary, 2500 University Avenue, N.W., T2N 1N4, Calgary, Alberta, Canada

    John I. Spicer & Brian R. McMahon

  2. Bamfield Marine Station, VOR 1B0, Bamfield, British Columbia, Canada

    John I. Spicer & Brian R. McMahon

Authors
  1. John I. Spicer
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  2. Brian R. McMahon
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Spicer, J.I., McMahon, B.R. Haemocyanin oxygen binding and the physiological ecology of a range of talitroidean amphipods (Crustacea) II. Effect of freezing, inorganic ions, and urate on O2 binding in vitro. J Comp Physiol B 161, 217–223 (1991). https://doi.org/10.1007/BF00262887

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  • DOI: https://doi.org/10.1007/BF00262887

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