Abstract
Catecholamine (CA) response to hypoxic exercise has been investigated during severe hypoxia. However, altitude training is commonly performed during mild hypoxia at submaximal exercise intensities. In the present study we tested whether submaximal exercise during mild hypoxia compared to normoxia leads to a greater increase of plasma concentrations of CA and whether plasma concentration of catecholamine sulphates change in parallel with the CA response. A group of 14 subjects [maximal oxygen uptake, 62.6 (SD 5.2) ml · min−1 · kg−1 body mass] performed two cycle ergometer tests of 1-h duration at the same absolute exercise intensities [191 (SD 6) W] during normoxia (NORM) and mild hypoxia (HYP) followed by 30 min of recovery during normoxia. Mean plasma concentrations of noradrenaline ([NA]), adrenaline ([A]), and noradrenaline sulphate ([NA-S]) were elevated (P < 0.01) after HYP and NORM compared with mean resting values and were higher after HYP [20.9 (SEM 3.1), 2.2 (SEM 0.24), 8.12 (SEM 1.5) nmol · 1−1, respectively] than after NORM [(13.7 (SEM 0.9), 1.5 (SEM 0.14), 6.8 (SEM 0.7) nmol · 1−1, respectively P < 0.01]. The higher plasma [NA-S] after HYP (P < 0.05) were still measurable after 30 min of recovery. From our study it was concluded that exercise at the same absolute submaximal exercise intensity during mild hypoxia increased plasma CA to a higher extent than during normoxia. Plasma [NA-S] response paralleled the plasma [NA] response at the end of exercise but, in contrast to plasma [NA], remained elevated until 30 min after exercise.
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References
Abenhaim L, Romain Y, Kuchel O (1981) Platelet phenolsulfotransferase and catecholamines: physiological and pathological variations in humans. Can J Physiol Pharmacol 59:300–306
Anderson RJ, Weinshilboum RM (1980) Phenolsulfotransferase in human tissue: radiochemical enzymatic assay and biochemical properties. Clin Chim Acta 103:79–90
Borg G (1970) Perceived exertion as an indicator of somatic stress. Scand J Rehabil Med 3:92–98
Bouissou P, Guezennec CY, Defer G, Pesquies P (1987) Oxygen consumption, lactate accumulation, and sympathetic response during prolonged exercise under hypoxia. Int J Sports Med 8:266–269
Chamberlain KG, Pestell RG, Best JD (1990) Platelet catecholamine contents are cumulative indexes of sympathoadrenal activity. Am J Physiol 259:E141-E147
Cleroux J, Peronnet F, Champlain J de (1983) Free and conjugated catecholamines in plasma and erythrocytes during exercise and following recovery (abstract). Med Sci Sports Exerc 15:95
Cleroux J, Peronnet F, Champlain J de (1985) Free and conjugated catecholamines in plasma and erythrocytes in normotensive and labile hypertensive subjects during exercise and recovery. J Hypertens 3 [Suppl]:S85-S88
Cuche JL, Safar M (1992) Potential role of the renal tubule fixing the level of plasma sulfoconjugated catecholamines in the dog. J Lab Clin Med 119:391–396
Davidson L, Vandongen R, Beilin LJ (1981) Effect of eating bananas on plasma free and sulfate-conjugated catecholamines. Life Sci 29:1773–1778
Escourrou P, Johnson DG, Rowell LB (1984) Hypoxemia increases plasma catecholamine concentrations in exercising humans. J Appl Physiol 57:1507–1511
Flamm SD, Taki J, Moore R, Lewis SF, Keech F, Maltais F, Ahmad M, Callahan R, Dragotakes S, Alpert N et al. (1990) Redistribution of regional and organ blood volume and effect on cardiac function in relation to upright exercise intensity in healthy human subjects. Circulation 81:1550–1559
Kjaer M, Bangsbo J, Lortie G, Galbo H (1988) Hormonal response to exercise in humans: influence of hypoxia and physical training. Am J Physiol 254:R197-R203
Kjaer M (1992) Exercise effects on adrenergic regulation of energy metabolism. In: Lamb DR, Gisolfi CV (eds) Perspectives in exercise science and sports medicine, vol. 5 Energy metabolism in exercise and sports. Brown and Benchmark, pp 345–376
Leuenberger U, Gleeson K, Wroblewski K, Prophet S, Zelis R, Zwillich C, Sinoway L (1991) Norepinephrine clearance is increased during acute hypoxemia in humans. Am J Physiol 261:111659–111664
Mazzeo RS, Bender PR, Brooks GA, Butterfield GE, Groves BM, Sutton JR, Wolfel EG, Reeves JT (1991) Arterial catecholamine responses during exercise with acute and chronic high-altitude exposure. Am J Physiol 261:E419-E424
Ratge D, Gehrke A, Melzner I, Wissel H (1986) Free and conjugated catecholamines in human plasma during physical exercise. Clin Exp Pharmacol Physiol 13:543–553
Rivett AJ, Eddy BJ, Roth JA (1982) Contribution of sulphate conjugated, deamination, and O-methylation to metabolism of dopamine and norepinephrine in human brain. J Neurochem 39:1009–1016
Rorie DK, Tyce GM (1987) Free and conjugated catecholamines in dog pulmonary artery: presence and pharmacological action. Am J Physiol 253:1166–1174
Rowell LB, Blackmon JR, Kenny MA, Escourrou P (1984) Splanchnic vasomotor and metabolic adjustments to hypoxia and exercise in humans. Am J Physiol 247:11251–11258
Sagnol M, Claustre J, Cottet-Emard JM, Pequignot JM, Fellmann N, Coudert J, Peyrín (1990) Plasma free and sulphated catecholamines after ultra-long exercise and recovery. Eur J Appl Physiol 60:91–97
Sothman MS, Gustafson AB, Chandler M (1987) Plasma free and sulfoconjugated catecholamine responses to varying exercise intensity. J Appl Physiol 63:654–658
Sothmann MS, Blaney J, Woulfe T, Donahue-Fuhrman S, Lefever K, Gustafson AB, Murthy VS (1990) Plasma free and sulfoconjugated catecholamines during sustained exercise. J Appl Physiol 68:452–456
Strobel G, Weicker H (1991) Catecholamine sulfates as internal standards in HPLC determinations of sulfoconjugated catecholamines in plasma and urine. Clin Chem 37:196–199
Strobel G, Werle E, Helfinger H, Griebel D, Weicker H (1988) Syntheses of the sulfoconjugated isomers of norepinephrine and dopamine, controlled by HPLC with ultraviolet detection, Eur J Biochem 176:397–402
Strobel G, Werle E, Weicker H (1990) Isomer specific kinetics of dopamine β-hydroxylase and arylsulfatase towards catecholamine sulfates. Biochem Int 20:343–351
Strobel G, Hack V, Weicker H (1993) Sustained noradrenaline sulphate response in long-distance runners and untrained subjects up to 2 h after exhausting exercise. Eur J Appl Physiol 66:421–426
Strobel G, Friedmann B, Jost J, Bärtsch P (1994) Plasma and platelet catecholamine sulfate response to various exercise tests. Am J Physiol 267:E537-E543
Unger T, Bun NT, Kuchel O, Schürch W (1980) Conjugated dopamine: peripheral origin, distribution, and response to acute stress in the dog. Can J Physiol Pharmacol 58:22–27
Wanger JA, Miles DS, Horvath SM (1980) Physiological adjustments of women to prolonged work during acute hypoxia. J Appl Physiol 49:367–373
Williams J, Powers S, Stuart M (1986) Hemoglobin desaturation in highly trained athletes during heavy exercise. Med Sci Sports Exerc 18:168–173
Wong KP, Khoo B, Tan TMC, Sit KH (1990) Phenolsulfotransferase (PST) and PAPS in catecholamine metabolism in human tissues: an overview. J Neural Trans 29 [Suppl]:163–171
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Strobel, G., Neureither, M. & Bärtsch, P. Effect of acute mild hypoxia during exercise on plasma free and sulphoconjugated catecholamines. Europ. J. Appl. Physiol. 73, 82–87 (1996). https://doi.org/10.1007/BF00262813
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DOI: https://doi.org/10.1007/BF00262813