Applied Microbiology and Biotechnology

, Volume 31, Issue 2, pp 158–162 | Cite as

Low temperatures stabilize interferon α-2 against proteolysis in Methylophilus methylotrophus and Escherichia coli

  • Julian A. Chesshyre
  • Alan R. Hipkiss
Applied Genetics and Regulation


The accumulation of interferon (IFN) α-2 in transformed strains of Escherichia coli and Methylophilus methylotrophus was greater at 25° C than at 37° C. Interferon α-2 catabolism was followed by measuring the change in IFN titre (measured immunoreactively) with time at temperatures between 25° C and 37° C in chloramphenicol-treated cells. The IFN α-2 titre remained constant at 29° C and below, while at higher temperatures the titres declined. The t1/2 values for IFN α-2 decreased with increasing incubation temperature. Pulse-chase studies using [35S]methionine, sodium dodecyl sulphate-gel electrophoresis and autoradiography demonstrated that IFN α-2 was subjected to degradation at 37° C while at 25° C it was stable. It is proposed that the susceptibility of IFN α-2 to degradation in both E. coli and M. methylotrophus is affected by incubation temperature and 30° C may be a transition temperature above which the conformation of the molecule is recognised by the bacterial proteases.


Sodium Escherichia Coli Electrophoresis Transition Temperature Interferon 
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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Julian A. Chesshyre
    • 1
  • Alan R. Hipkiss
    • 1
  1. 1.Department of Biochemistry, King's College LondonUniversity of LondonLondonUnited Kingdom

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