Summary
The distribution of acid mucopolysaccharides in bladder epithelium was studied by colloidal iron staining in experimental cystitis of rats. In normal bladders the staining was mainly confined to the superficial (luminal) layer, but observed uniformly in all layers of epithelium in cystitis. Enzyme digestion (hyaluronidase, heparitinase, chondroitinase AC, chondroitinase ABC) disclosed the existence of hyaluronic acid, heparan sulphate and dermatan sulphate in the bladder epithelium. The possible roles of these mucopolysaccharides glycosaminogycans) were discussed.
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References
Allalouf D, Ber A, Rechnic J, Sharon N (1967) Anticoagulant properties of an acid mucopolusaccharide preparation isolated from rat kidney. Thromb Diath Haemorrh 17:264–272
Castor CW, Greene JA (1968) Regional distribution of acid mucopolysaccharides in the kidney. J Clin Invest 47:2125–2132
Fukushi Y, Orikasa S, Kagayama M (1979) An electron microscopic study of the interaction between vesical epithelium and E. coli. Invest Urol 17:61–68
Hanno PM, Fritz R, Wein AJ, Mulholland SG (1978) Heparin as antibacterial agent in rabbit bladder. Urology 12:411–415
Hanno PM, Parsons CL, Shrom SH, Fritz R, Mulholland SG (1978) The protective effect of heparin in experimental bladder infection. J Surg Res 25:324–329
Levin S, Richter WR (1975) Ultrastructure of cell surface coat (Glycocalyx) in rat urinary bladder epithelium. Cell Tissue Res 158:281–283
McManas JFA, Mowry FW (1960) Staining methods. Hoeber, New York, p 135
Monis B, Dorfman HD (1967) Some histochemical observation on transitional epithelium of man. J Histochem Cytochem 15:475–481
Ototani N, Yoshizawa Z (1979) Purification of mucopolysaccharides by affinity chromatography using glycosaminoglycan bound AH-sephrose 4B. Schauer R, Boer P, Buddecke E, Kramer MF, Vliegenthard JFG, Wiegant H (eds) Proceedings of the fifth international symposium of glycoconjugates Kiel. Thieme Stuttgart, p. 388–389
Parson CL, Greenspan C, Mulholland SG (1975) The primary antibacterial defense mechanism of the bladder. Invest Urol 13:72–76
Parson CL, Greenspan C, Moore SW, Mulholland SG (1977) Role of surface mucin in primary antibacterial defense of bladder. Urology 9:48–52
Robinson RHOB (1960) The significance of abnormality of the volume and of the composition of the urine. Alken CE, Dix VW, Weyrauch HM, Wildbolz E (eds) Encyclopedia of Urology, vol 3. Springer, Berlin Göttingen Heidelberg, p. 28
Takeoka S (1972) Histochemical experimental methods for mucopolysaccharides (Japanese). Kagaku no Ryoiki 96:263–278
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Kuwahara, M., Tokiwa, M. & Orikasa, S. Bacterial infection and acid mucopolysaccharides in epithelium of rat urinary bladder. Urol. Res. 10, 93–96 (1982). https://doi.org/10.1007/BF00262410
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DOI: https://doi.org/10.1007/BF00262410