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Urinary unconjugated 5α-androstane-3α, 17β-diol in patients with prostatic tumours

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Summary

A sensitive and reliable radioimmunoassay (RIA) for urinary unconjugated 5α-androstane-3α, 17β-diol is described. The mean overall recovery of unconjugated 5α-androstane-3α, 17β-diol was found to be 57.4%. The sensitivity of the assay was 79 fmol per assay tube and the intra and inter-assay variations ranged between 7.2% and 11.4%. The mean ± SEM for the concentration of this androgen in the urine of normal men was 339.6±66.8 nmol/24h. The corresponding values for patients with benign prostatic hypertrophy (BHP) and carcinoma of the prostate (Ca) were 297.8±44.7 and 1592.1±622.7 respectively. The mean value for Ca patients was significantly higher than either BPH (p<0.05) or normal subject (p<0.02), suggesting a differential urinary excretion pattern for unconjugated 5α-androstane-3α, 17β-diol between BPH and Ca patients. It is concluded that the combined measurement of this androgen in the plasma and urine provides a more accurate assessment of the profile of this hormone than a single plasma estimation.

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Authors and Affiliations

  1. Prostate Research Laboratory, Royal Postgraduate Medical School, UK

    C. M. Puah, G. Williams &  R. Ghanadian

  2. The Institute of Urology, University of London, UK

    C. M. Puah, G. Williams &  R. Ghanadian

Authors
  1. C. M. Puah
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  2. G. Williams
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  3. R. Ghanadian
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Puah, C.M., Williams, G. & Ghanadian, R. Urinary unconjugated 5α-androstane-3α, 17β-diol in patients with prostatic tumours. Urol. Res. 10, 81–84 (1982). https://doi.org/10.1007/BF00262407

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  • DOI: https://doi.org/10.1007/BF00262407

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