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Urological Research

, Volume 13, Issue 2, pp 79–84 | Cite as

Mannose inhibition of Escherichia coli adherence to urinary bladder epithelium: Comparison with yeast agglutination

  • M. R. Ruggieri
  • P. M. Hanno
  • R. M. Levin
Originals

Abstract

The adherence of piliated strains of Escherichia coli (E. coli) to mammalian epithelial cells has been reported by several investigators to be specifically inhibited by D(+)-mannose or its derivatives. Much of this work utilized mannose type compounds to inhibit agglutination of mannan containing yeast cells by E. coli to demonstrate mannose sensitivity. This report investigates the ability of the neotype strain of E. coli (which is sensitive to mannose inhibition of yeast cell agglutination) to bind and metabolize radiolabeled D(+)-mannose. In addition the relative efficacy of D(+)-mannose and heparin to inhibit the adherence of E. coli to rabbit bladder mucosa was compared. Results showed that although D(+)-mannose did block E. coli-yeast cell agglutination in a reversible manner, radiolabeled D(+)-mannose binding by E. coli could not be displaced by 1,000 fold excess unlabeled D(+)-mannose. This suggests uptake of the sugar as opposed to a surface binding phenomenon which was confirmed by the demonstration of significant metabolism of mannose by E. coli. The same concentration of D(+)-mannose which prevented E. coli-yeast cell agglutination was not particularly effective in preventing E. coli adherence to the acid denuded rabbit bladder. Heparin treatment of the acid denuded bladder was very effective in preventing E. coli adherence but was ineffective in preventing E. coli-yeast cell agglutination. This indicates that E. coli-yeast cell agglutination should not be correlated with E. coli adherence to mammalian epithelial tissue.

Key words

E. coli adherence Mannose binding Yeast agglutination Urinary bladder Urinary tract infection 

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References

  1. 1.
    Aronson M, Medalia O, Schori L, Mirelman D, Sharon N, Ofek I (1979) Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl α-d-mannopyranoside. Infect Dis 139:329–332Google Scholar
  2. 2.
    Avots-Avotins AE, Fader RC, Davis CP (1981) Environmental alteration and two distinct mechanisms of E. coli adherence to bladder epithelial cells. Invest Urol 18:364–370Google Scholar
  3. 3.
    Duguid JP, Old DC (1980) Adhesive properties of Enterobacteriaceae. In: Beachy EH (ed) Bacterial adherence. Receptors and recognition. Chapman and Hall, New York, Series B, vol 6, pp 197–198Google Scholar
  4. 4.
    Eshdat Y, Ofek I, Yashouv-Gan Y, Sharon N, Mirelman D (1978) Isolation of a mannose-specific lectin from Escherichia coli and its role in the adherence of the bacteria to epithelial cells. Biochem Biophys Res Commun 85:1551–1559Google Scholar
  5. 5.
    Fader RC, Avots-Avotins AE, Davis CP (1979) Evidence for Pili — mediated adherence of Klebsiella pneumoniae to rat bladder epithelial cells in vitro. Infect Immun 25:729–737Google Scholar
  6. 6.
    Hanno PM, Fritz R, Wein AJ, Mulholland SG (1978) Heparin as antibacterial agent in rabbit bladder. Urology 12:441–415Google Scholar
  7. 7.
    Hanno PM, Parsons CL, Shrom SH, Fritz R, Mulholland SG (1978) The protective effect of heparin in experimental bladder infection. J Surg Res 25:324–329Google Scholar
  8. 8.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement using the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  9. 9.
    Ofek I, Beachy EH (1978) Mannose binding and epithelial cell adherence of Escherichia coli. Infect Immun 22:247–254Google Scholar
  10. 10.
    Ofek I, Beachy H, Sharon N (1978) Surface sugars of animal cells as determinants of recognition in bacterial adherence. Trends Biol Sci 0:159–160Google Scholar
  11. 11.
    Ofek I, Mirelman D, Sharon N (1977) Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature 265:623–625Google Scholar
  12. 12.
    Ofek I, Mosek A, Sharon N (1981) Mannose-specific adherence of Escherichia coli freshly excreted in the urine of patients with urinary tract infections, and of isolates subcultured from the infected urine. Infect Immun 34:708–711Google Scholar
  13. 13.
    Parsons CL, Mulholland SG, Anwar H (1979) Antibacterial activity of bladder surface mucin duplicated by exogenous glycosaminoglycan (heparin). Infect Immun 24:552–557Google Scholar
  14. 14.
    Schaffer AJ, Amundsen SK, Jones JM (1980) Effect of carbohydrates on adherence of Escherichia coli to human urinary tract cells. Infect Immun 30:531–537Google Scholar
  15. 15.
    Sharon N, Eshdat Y, Silverblatt FJ, Ofek I (1981) Bacterial adherence to cell surface sugars. In: Adhesion and Microorganism Pathogenicity. Pitman Medical, Tunbridge Wells (Ciba Foundation symposium), pp 119–141Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. R. Ruggieri
    • 1
    • 2
    • 3
  • P. M. Hanno
    • 1
    • 2
    • 3
  • R. M. Levin
    • 1
    • 2
    • 3
  1. 1.Department of PharmacologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Division of UrologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  3. 3.Philadelphia Veterans Administration Medical CenterPhiladelphiaUSA

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