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Effect of water activity on the growth and the production of cAMP phosphodiesterase inhibitor with Bacillus subtilis

  • Applied Microbiology
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Summary

Bacillus subtilis C-756, a producer of cyclic adenosine 3′,5′-monophosphate (cAMP) phosphodiesterase inhibitor, was cultured in media adjusted to various water activity (aw) levels by addition of three different solutes, sodium chloride, ethylene glycol and polyethylene glycol 1540 (PEG).

B. subtilis C-756 can grow, however weakly, at aw levels of 0.94 and 0.93.

The presence of all three solutes in the medium inhibited growth, cell mass as well as inhibitor production. PEG was found to be most inhibitory, but the effect can not be explained in terms of a decreased water activity in the medium. It is rather the increased viscosity of the medium, which results in a decreased oxygen transfer rate.

Comparing ethylene glycol and sodium chloride, the presence of ethylene glycol appears to favour inhibitor production, whereas sodium chloride favours cell mass production.

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Authors and Affiliations

  1. Agency of Industrial Science and Technology, Fermentation Research Institute, Yatabe-machi, Ibaraki, Japan

    Kuniaki Hosono

  2. Applied Microbiology, Chemical Center, University of Lund, P.O. Box 124, S-22100, Lund, Sweden

    Bärbel Hahn-Hägerdal

Authors
  1. Kuniaki Hosono
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  2. Bärbel Hahn-Hägerdal
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Hosono, K., Hahn-Hägerdal, B. Effect of water activity on the growth and the production of cAMP phosphodiesterase inhibitor with Bacillus subtilis . Appl Microbiol Biotechnol 24, 224–229 (1986). https://doi.org/10.1007/BF00261541

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  • DOI: https://doi.org/10.1007/BF00261541

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