Summary
Two high-molecular-weight subunit (HMWS) glutenin genes from the A and B genomes of the hexaploid bread wheat Triticum aestivum L. cv Cheyenne have been isolated and sequenced. Both of these genes are of the high Mr class (x-type) of HMW glutenins, and have not been previously reported. The entire set of six HMW genes from cultivar Cheyenne have now been isolated and characterized. An analysis of the Ax and Bx sequences shows that the Ax sequence is similar to the homoeologous gene from the D genome, while the Bx repeat structure is significantly different. The repetitive region of these proteins can be modelled as a series of interspersed copies of repeat modifs of 6, 9, and 15 amino acid residues. The evolution of these genes includes single-base substitutions over the entire coding region, plus insertion/deletions of single or blocks of repeats in the central repetitive domain.
Similar content being viewed by others
References
Anderson OD, Litts JC, Gautier M-F, Greene FC (1984) Nucleic acid sequence and chromosome assignment of a wheat storage protein gene. Nucleic Acid Res 12:8129–8144
Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462
Bartels D, Altosaar I, Harberd NP, Barker RF, Thompson RD (1986) Molecular analysis of γ-gliadin gene families at the complex locus of bread wheat (T. aestivum L.). Theor Appl Genet 72:845–853
Branlard G, Dardevet M (1985) Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354
Colot V, Robert LS, Kavanagh TA, Bevan MW, Thompson RD (1987) Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco. EMBO J 6:3559–3564
Dale RMK, McClure BA, Houchins JP (1985) A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA. Plasmid 13:31–40
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J (1986) mRNA Transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucleic Acids Res 14:2229–2240
Ewart JAD (1968) A hypothesis for the structure and rheology of glutenin. J Sci Food Agric 19:617–623
Forde J, Forde BG, Fry RP, Kreis M, Shewry PR, Miflin BJ (1983) Identification of barley and wheat cDNA clones related to the high-Mr polypeptides of wheat gluten. FEBS Lett 162:360–366
Forde J, Malpica J-M, Halford NG, Shewry PR, Anderson OD, Greene FC, Miflin BJ (1985) The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Res 13:6817–6832
Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Galili G, Feldman M (1983) Genetic control of endosperm proteins in wheat: 2. Variation in high molecular weight glutenin and gliadin subunits of Triticum aestivum. Theor Appl Genet 66:77–86
Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126
Harberd NP, Bartels D, Thompson RD (1986) DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits in wheat. Biochem Genet 24:579–596
Harberd NP, Flavell RB, Thompson RD (1987) Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet 209:326–332
Kozak M (1987) An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acid Res 15:8125–8148
Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those for wheat, barely and rye. Oxford Surv Plant Mol Cell Biol 2:253–317
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Mead DA, Szczesna-Skorupa E, Kemper B (1986) Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng 1:67–74
Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nucleic Acids Res 9:309–321
Payne PI, Harris PA, Law CN, Holt LM, Blackman JA (1980) The high-molecular-weight subunits of glutenin: structure, genetics and relationship to bread-making quality. Ann Technol Agric 29:309–320
Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 1: allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236
Payne PI, Holt LM, Worland AG, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 3: telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138
Payne PI, Holt LM, Thompson RD, Bartels D, Harberd NP, Harris PA, Law CN (1983) The high-molecular-weight glutenins: classical genetics, molecular genetics and the relationship to bread-making quality. Proc 6th Int Wheat Genet Symp 827–834
Payne PI, Holt LM, Jackson EA, Law CN (1984) Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Philos Trans R Soc London Ser B 304:359–371
Sanchez-Pescador R, Urdea MS (1984) Use of unpurified primers in sequencing. DNA 3:339–343
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. PNAS 74:5463–5467
Shewry PR, Field JM, Faulks AJ, Parmar S, Miflin BJ, Dietler MD, Lew E, Kasarda DD (1984) The purification and n-terminal amino acid sequence analysis of the high molecular weight gluten polypeptides of wheat. Biochim Biophy Acta 788:23–34
Sugiyama T, Rafalski A, Peterson D, Söll D (1985) A wheat HMW glutenin gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737
Tatham AS, Shewry PR, Miflin BJ (1984) Wheat gluten elasticity: a similar molecular basis to elastin? FEBS Lett 177:205–208
Thompson RD, Bartels D, Harberd NP, Flavell RB (1983) Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones. Theor Appl Genet 67:87–96
Thompson RD, Bartels D, Harberd NP (1985) Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit. Nucleic Acids Res 13:6833–6846
Author information
Authors and Affiliations
Additional information
Communicated by J. Beckmann
Rights and permissions
About this article
Cite this article
Anderson, O.D., Greene, F.C. The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theoret. Appl. Genetics 77, 689–700 (1989). https://doi.org/10.1007/BF00261246
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00261246