Abstract
A SORB-GEL method has been worked out for analysing 99mTc-labelled compounds which (in a few minutes) enables the pertechnetate content to be determined in a preparation. The method is based upon a different tupe of behaviour of 99mTc-labelled compound, pertechnetate in the columns packed with Sephadex G-10, and with alumina during elution with saline.
Polythene syringes 4.7 cm long and 1 cm in diameter were used as chromotographic columns. A syringe packed with Sephadex G-10 transferred 0.1 ml of 99mTc-labelled compound into the column, and the activity of the column was then measured in the ionisation chamber. Using a syringe, 20 ml of saline was foreced through this column. The eluate was then removed. Using an injection needle, a second column, packed with alumina, was connected with the first. Similarly, a third column, packed with Sephadex G-10, was connected to the second. Through all of them 40 ml of saline was forced. The third column containing Sephadex G-10 was then disconnected and its activity was measured in the ionisation chamber. The pertechnetate content in the preparation was then calculated from the measured values.
The method is suitable for determinating the free pertechnetate content in strong and weak chelate compounds and in particle preparations.
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Macháň, V., Vilček, Š., Kalinčák, M. et al. SORB-GEL method of analysis of 99mTc-labelled compounds. Eur. J. Nucl. Med. 5, 39–43 (1980). https://doi.org/10.1007/BF00261205
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DOI: https://doi.org/10.1007/BF00261205