Summary
The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans. In the presence of the merD gene, β-galactosidase activity was 2- to 4-fold lower. The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.
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References
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Communicated by H. Böhmc
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Nucifora, G., Silver, S. & Misra, T.K. Down regulation of the mercury resistance operon by the most promoter-distal gene merD . Molec. Gen. Genet. 220, 69–72 (1989). https://doi.org/10.1007/BF00260858
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DOI: https://doi.org/10.1007/BF00260858