Skip to main content
SpringerLink
Log in

A mutation of Streptomyces lividans which prevents intraplasmid recombination has no effect on chromosomal recombination

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

A mutation (rec-46) of Streptomyces lividans, previously shown to prevent (or greatly diminish) homologous and illegitimate intraplasmid recombination, was shown to have no effect on generalised chromosomal recombination occurring in matings or in protoplast fusions, nor to affect homologous recombination between a recombinant plasmid and the host chromosome. By comparison with Escherichia coli mutants defective in various aspects of recombination, the rec-46 mutation is similar to those in recF, recJ, recO and topA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beck E, Ludwig G, Auerswald EA, Reis B, Schaller H (1982) Nucleotide sequence and exact localisation of the neomycin phosphotransferase gene from transposon Tn5. Gene 19:327–336

    Google Scholar 

  • Bullock P, Champoux JJ, Botchan M (1985)Association of crossover points with topoisomerase cleavage sites: a model for nonhomologous recombination. Science 230:954–958

    CAS  PubMed  Google Scholar 

  • Chen CW, Tsai JF-Y, Chuang S-E (1987) Intraplasmid recombination in Streptomyces lividans 66. Mol Gen Genet 209:154–158

    Google Scholar 

  • Clark AJ (1973) Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet 7:67–86

    Google Scholar 

  • Cohen A, Laban A (1983) Plasmidic recombination in Escherichia coli K-12: The role of recF gene function. Mol Gen Genet 189:471–474

    Google Scholar 

  • Deng Z, Kieser T, Hopwood DA (1988) “Strong incompatibility” between derivatives of the Streptomyces multi-copy plasmid pIJ101. Mol Gen Genet 214:286–294

    Google Scholar 

  • Dressler D, Potter H (1982) Molecular mechanisms in genetic recombination. Annu Rev Biochem 51:727–761

    Google Scholar 

  • Fishel RA, Kolodner R (1984) Escherichia coli strains containing mutations in the structural gene for topoisomerase I are recombination deficient. J Bacteriol 160:1168–1170

    Google Scholar 

  • Harold RJ, Hopwood DA (1970a) Ultraviolet-sensitive mutants of Streptomyces coelicolor. I. Phenotypic characterisation. Mutat Res 10:427–438

    Google Scholar 

  • Harold RJ, Hopwood DA (1970b) Ultraviolet-sensitive mutants of Streptomyces coelicolor. II. Genetics. Mutat Res 10:439–448

    Google Scholar 

  • Harold RJ, Hopwood DA (1972) A rapid method for complementation testing of ultraviolet-sensitive (uvs) mutants of Streptomyces coelicolor. Mutat Res 16:27–34

    Google Scholar 

  • Hayes JB, Boehmer S (1978) Antagonists of DNA gyrase inhibit repair and recombination of UV irradiated phage. Proc Natl Acad Sci USA 75:4125–4129

    Google Scholar 

  • Henderson DJ, Lydiate DJ, Hopwood DA (1989) Structural and functional analysis of the mini-circle, a transposable element of Streptomyces coelicolor A3(2). Mol Microbiol 3:1307–1318

    Google Scholar 

  • Hopwood DA, Kieser T, Wright HM, Bibb MJ (1983) Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol 129:2257–2269

    Google Scholar 

  • Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985) Genetic manipulation of Streptomyces. A laboratory manual. John Innes Foundation, Norwich

    Google Scholar 

  • Ikeda H, Moriya K, Matsumoto T (1980) In vitro study of illegitimate recombination: Involvement of DNA gyrase. Cold Spring Harbor Symp Quant Biol 45:399–402

    Google Scholar 

  • Ikeda H, Aoki K, Naito A (1982) Illegitimate recombination mediated in vitro by DNA gyrase of Escherichia coli: Structure of recombinant DNA molecules. Proc Natl Acad Sci USA 79:3724–3728

    Google Scholar 

  • James AA, Morrison PT, Kolodner R (1982) Genetic recombination of bacterial plasmid DNA — Analysis of the effect of recombination-deficient mutations on plasmid recombination. J Biol 160:411–430

    Google Scholar 

  • Katz E, Thompson CJ, Hopwood DA (1983) Cloning and expression of the tyrosinase gene from Streptomyces antihioticus in Streptomyces lividans. J Gen Microbiol 129:2703–2714

    Google Scholar 

  • Kieser T, Hopwood DA, Wright HM, Thompson CJ (1982) pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet 185:223–238

    Google Scholar 

  • Kolodner R, Fishel RA, Howard M (1985) Genetic recombination of bacterial plasmid DNA: Effect of recF pathway mutations on plasmid recombination in Escherichia coli. J Bacteriol 163:1060–1066

    Google Scholar 

  • Lomovskaya ND, Mkrtumian NM, Gostimskaya NL, Danilenko VN (1972) Characterisation of temperate actinophage ϕ C31 isolated from Streptomyces coelicolor A3(2). J Virol 9:258–262

    Google Scholar 

  • Lydiate DJ, Ikeda H, Hopwood DA (1986) A 2.6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element. Mol Gen Genet 203:79–88

    Google Scholar 

  • Lydiate DJ, Ashby AM, Henderson DJ, Kieser HM, Hopwood DA (1989) Physical and genetic characterization of chromosomal copies of the Streptomyces coelicolor mini-circle. J Gen Microbiol 135:941–955

    Google Scholar 

  • Matsushima P, Baltz RH (1987) recA gene of Escherichia coli complements defects in DNA repair and mutagenesis in Streptomyces fradiae JS6 (mcr-6). J Bacteriol 169:4834–4836

    Google Scholar 

  • Murty VV, Madiraju S, Templin A, Clark AJ (1988) Properties of a mutant recA-encoded protein reveal a possible role for Escherichia coli recF-encoded protein in genetic recombination. Proc Natl Acad Sci USA 85:6592–6596

    Google Scholar 

  • Raina JL, Ravin AW (1979) Superhelical DNA in Streptococcus sanguis: Role in recombination in vivo. Mol Gen Genet 176:171–181

    Google Scholar 

  • Ruther U, Koenen M, Otto K, Muller-Hill B (1981) pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Res 9:4087–4098

    Google Scholar 

  • Smith GR (1988) Homologous recombination in procaryotes. Microbiol Rev 52:1–28

    Google Scholar 

  • Soberon X, Covarrubias L, Bolivar F (1980) Construction and characterisation of new cloning vehicles, IV. Deletion derivatives of pBR322 and pBR325. Gene 9:287–305

    Google Scholar 

  • Sternglanz R, DiNardo S, Voelkel KA, Nishimura Y, Hirota Y, Bucherer K, Zumstein L, Wang JC (1981) Mutations in the gene coding for Escherichia coli topoisomerase I affect transcription and transposition. Proc Natl Acad Sci USA 78:2747–2751

    Google Scholar 

  • Stonesifer J, Baltz RH (1985) Mutagenic DNA repair in Streptomyces. Proc Natl Acad Sci USA 82:1180–1193

    Google Scholar 

  • Tsai JF-Y, Chen CW (1987) Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination. Mol Gen Genet 208:211–218

    Google Scholar 

  • Volkert MR, Hartke MA (1984) Suppression of Escherichia coli recF mutations by recA-linked srfA mutations. J Bacteriol 157:498–506

    Google Scholar 

  • Yi T-M, Stearns D, Demple B (1988) Illegitimate recombination in an Escherichia coli plasmid: modulation by DNA damage and a new bacterial gene. J Bacteriol 170:2898–2903

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. John Innes Institute and AFRC Institute of Plant Science Research, Norwich, UK

    Helen M. Kieser, Duncan J. Henderson & David A. Hopwood

  2. Institute of Microbiology and Immunology and Institute of Genetics, National Yang-Ming Medical College, Taipei, Taiwan, ROC

    Carton W. Chen

Authors
  1. Helen M. Kieser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Duncan J. Henderson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carton W. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David A. Hopwood
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by B.J. Kilbey

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kieser, H.M., Henderson, D.J., Chen, C.W. et al. A mutation of Streptomyces lividans which prevents intraplasmid recombination has no effect on chromosomal recombination. Molec. Gen. Genet. 220, 60–64 (1989). https://doi.org/10.1007/BF00260856

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00260856

Key words

Search

Navigation