Summary
DNA sequences from two spontaneous mutations of Drosophila melanogaster associated with insertion of a Doc transposable element have been cloned. In white-one, the element is inserted in the white locus close to where transcription initiates. In a lethal allele of suppressor of forked, su(f)S2, the element is inserted within the transcription unit in the protein coding region. Four other Doc elements have been cloned from a wild-type strain. Doc is a member of the class of transposable elements known as retroposons, which includes the D. melanogaster F, G, Jockey, and I elements. There is no sequence homology between the ends of the Doc element. The 3′ or right end terminates with a polyadenylation signal sequence followed by a stretch of oligo-A. The length of the oligo-A varies between elements, and a duplication of variable size is found as a direct repeat flanking inserted Doc elements. Members of the family are conserved at the 3′ end, but may be truncated at the 5′ or left end. These structural features suggest a mechanism of transposition via an RNA intermediate.
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Bender W, Akam M, Karch F, Beachy PA, Peifer M, Spierer P, Lewis EB, Hogness DS (1983) Molecular genetics of the bithorax complex of Drosophila melanogaster. Science 221:2329
Berger SL, Kimmel AR (1987) Guide to Molecular Cloning Techniques. Methods Enzymol 152
Di Nocera PP (1988) Close relationship between non-viral retroposons in Drosophila melanogaster. Nucleic Acids Res 16:4041–4052
Di Nocera PP, Digan ME, David I (1983) A family of oligo-adenylated transposable sequences in Drosophila melanogaster. J Mol Biol 168:715–727
Levis R, Bingham PM, Rubin GM (1982) Physical map of the white locus of Drosophila melanogaster. Proc Natl Acad Sci USA 79:564–568
Lindsley DL, Grell EH (1968) Genetic Variations of Drosophila melanogaster. Publication 627, Carnegie Inst Wash, DC
Mizrokhi LJ, Georgeiva SG, Ilyin YV (1988) jockey, a mobile Drosophila element similar to mammalian LINES, is transcribed from the internal promoter by RNA polymerase 11. Cell 54:685–691
Morgan TH (1910) Sex limited inheritance in Drosophila. Science 32:120–122
Morgan TH, Bridges CB (1916) Sex-linked inheritance in Drosophila. Publication 237, Carnegie Inst Wash, DC
O'Hare K, Murphy C, Levis R, Rubin GM (1984) DNA sequence of the white locus of Drosophila melanogaster. J Mol Biol 180:437–455
Pirrotta V, Brockl Ch (1984) Transcription of the white locus and some of its mutants. EMBO J 3:563–568
Priimagi AF, Mizrokhi LJ, Ilyin YV (1988) The Drosophila mobile element jockey belongs to LINES and contains coding sequences homologous to some retroviral proteins. Gene 70:253–262
Rogers JH (1985) The origin and evolution of retroposons. Int Rev Cytol 93:188–280
Sang HM, Pelisson A, Bucheton A, Finnegan DJ (1984) Molecular lesions associated with white gene mutations induced by I-R hybrid dysgenesis in Drosophila melanogaster. EMBO J 3:3079–3085
Schalet A (1986) The distribution of an complementation relationships between spontaneous X-linked recessive lethal mutations recovered from crossing long-term laboratory stocks of Drosophila melanogaster. Mutat Res 163:115–144
Schneuwly S, Kuroiwa A, Gehring WJ (1987) Molecular analysis of the dominant homeotic antennapedia phenotype. EMBO J 6:201–206
Steller H, Pirrotta V (1985) Expression of the Drosophila white gene under the control of the hsp70 heat shock promoter. EMBO J 4:3765–3722
Toh H, Kikuno R, Hayashida H, Miyata T, Kugimiya W, Inouye S, Yuki S, Saigo K (1985) Close structural resemblance between putative polymerase of a Drosophila transposable genetic element 17.6 and pol gene product of Moloney murine leukaemia virus. EMBO J 4:1267–1272
Zachar Z, Bingham PM (1982) Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster. Cell 30:529–541
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Communicated by D.J. Finnegan
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Driver, A., Lacey, S.F., Cullingford, T.E. et al. Structural analysis of Doc transposable elements associated with mutations at the white and suppressor of forked loci of Drosophila melanognster . Molec. Gen. Genet. 220, 49–52 (1989). https://doi.org/10.1007/BF00260854
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DOI: https://doi.org/10.1007/BF00260854