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Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects

Theoretical and Applied Genetics volume 73, pages 625–629 (1987)Cite this article

Summary

One natural population (F0 generation) of Beta maritima situated on the French Atlantic coast has been analysed. It was composed of 62% female, 30% hermaphrodite and 8% intermediate plants. The analysis of half-sib progeny (F1 generation) obtained from in situ open pollination demonstrates the cytoplasmic determination of male sterility in Beta maritima and the restoration of fertility by nuclear genes. The mitochondrial DNA (mtDNA) and the chloroplast DNA (ctDNA) of sixteen F1 plants, extracted from offspring of the three sexual phenotypes, were analysed using the restriction enzymes Sal I and Bam HI, respectively. Two cytoplasmic lines with their own peculiar genetic characteristics were distinguished using the restriction enzyme patterns of mtDNA: (i) the S cytoplasmic line was found in segregating progeny of two F0 plants; all three phenotypes were produced (that is, progeny including hermaphrodite, female and intermediate plants); (ii) the N cytoplasmic line was found in the progeny of one F0 hermaphrodite plant; this produced only hermaphrodites. Thus, segregating and non-segregating hermaphrodite F0 plants can be distinguished. The nuclear genes maintaining sterility or restoring fertility are expressed in line S. At the same time the analysis of Beta vulgaris material has been carried out at the molecular level: N cytoplasmic lines of B. vulgaris and B. maritima differed only by 3 fragments of mtDNA; but the S cytoplasmic line of B. maritima was very different from Owen's cytoplasmic male sterile line of B. vulgaris. No variation in the ctDNA pattern was detected within and between the two taxa.

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Affiliations

  1. Laboratoire de Génétique Ecologique et Biologie des Populations Végétales, Université des Sciences et Techniques de Lille Flandres Artois, F-59655, Villeneuve d'Ascq Cedex, France

    V. Boutin, P. Saumitou-Laprade, R. Jean & Ph. Vernet

  2. Botanisches Institut der Universität Düsseldorf, Universitätsstrasse 1, D-4000, Düsseldorf 1, Federal Republic of Germany

    G. Pannenbecker, W. Ecke, G. Schewe & G. Michaelis

Authors
  1. V. Boutin
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  2. G. Pannenbecker
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  3. W. Ecke
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  4. G. Schewe
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  5. P. Saumitou-Laprade
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  6. R. Jean
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  7. Ph. Vernet
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  8. G. Michaelis
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Communicated by G. Wenzel

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Boutin, V., Pannenbecker, G., Ecke, W. et al. Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theoret. Appl. Genetics 73, 625–629 (1987). https://doi.org/10.1007/BF00260768

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  • DOI: https://doi.org/10.1007/BF00260768

Key words

  • Wild and cultivated sugar beet (Beta maritima, Beta vulgaris)
  • Cytoplasmic male sterility, nuclear restorer genes
  • Mitochondrial and chloroplast DNAs
  • Restriction endonuclease fragment analysis