Summary
The diaminopimelate (DAP) pathway provides the cell with lysine and with DAP, a vital cell wall constituent. Mutations in the DAP pathway of lysine biosynthesis are lethal for cells exposed to lysine in the absence of DAP. In this paper, the substitution of thedapD gene ofEscherichia coli with the kanamycin resistance gene from Tn903 is described and its possible uses are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H (1982) Nucleotide sequence and exact localization of the neomycin transferase gene from transposon Tn5. Gene 19:327–336
Bendiak DS, Friesen JD (1981) Organisation of genes in the four minute region of theEscherichia coli chromosome: evidence thatrpsB andtsf are co-transcribed. Mol Gen Genet 181:356–362
Bukhari AI, Taylor AL (1971) Genetic analysis of diaminopimelic acid- and lysine-requiring mutants ofEscherichia coli. J Bacteriol 105:844–854
Degryse E (1990) Stability of a host/vector system based on complementation of an essential gene inEscherichia coli. J Biotechnol, in press
Jasin M, Schimmel (1984) Deletion of an essential gene inEscherichia coli by site-specific recombination with linear DNA fragments. J Bacteriol 159:783–786
Lathe R, Bollen A, Herzog R (1981) Revised location of theEscherichia coli gene coding for ribosomal protein S2. Mol Gen Genet 182:178–179
Lathe R, Vilotte JL, Clark AJ (1987) Plasmid and bacteriophage vectors for excision of intact inserts. Gene 57:193–201
Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
Nakayama K, Kelly SM, Curtiss III R (1988) Construction of an asd+ expression-cloning vector: stable maintenance and high level expression of cloned genes in aSalmonella vaccine strain. Biotechnology 6:693–697
Richaud C, Richaud F, Martin C, Haziza C, Patte JC (1984) Regulation of expression and nucleotide sequence of theEscherichia coli dapD gene. J Biol Chem 259:14823–14828
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. second edn vols 1, 2, 3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Tessier LH, Jallat S, Sauvageot M, Crystal RG, Courtney M (1986) RNA structural elements for expression inEscherichia coli. FEES Lett 208:183–188
Author information
Authors and Affiliations
Additional information
Communicated by R. Devoret
Rights and permissions
About this article
Cite this article
Degryse, E. Development of stable, genetically well-defined conditionally viableEscherichia coli strains. Molec. Gen. Genet. 227, 49–51 (1991). https://doi.org/10.1007/BF00260705
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00260705