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Genetic analysis of a lactococcal plasmid replicon

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Summary

The sequence and genetic organization was determined of the 2508 by lactococcal portion of pFX2, which was derived from a crypticLactococcus lactis subsp.lactis plasmid and used as the basis for construction of a series of lactococcal vectors. A lactococcal plasmid plus origin and two replication protein-coding regions (repA andrepB) were located. RepA has a helix-turn-helix motif, a geometry typical of DNA-binding proteins. RepB shows a high degree of homology to the plasmid replication initiation proteins from other gram-positive bacteria andMycoplasma. The transcribed inverted repeat sequence betweenrepA andrepB could form an attenuator to regulate pFX2 replication. Upstream of theori site, and in a region which was non-essential for replication, a 215 by sequence identical to the staphylococcal plasmid pE194 and carrying the RSA site was identified. The genetic organization of this lactococcal plasmid replicon shares significant similarity with pE194 group plasmids.

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Authors and Affiliations

  1. Department of Biotechnology, Massey University, Palmerston North, New Zealand

    Fengfeng Xu & Pak-Lam Yu

  2. New Zealand Dairy Research Institute, Palmerston North, New Zealand

    Lindsay E. Pearce

Authors
  1. Fengfeng Xu
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  2. Lindsay E. Pearce
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  3. Pak-Lam Yu
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Communicated by W. Goebel

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Xu, F., Pearce, L.E. & Yu, PL. Genetic analysis of a lactococcal plasmid replicon. Molec. Gen. Genet. 227, 33–39 (1991). https://doi.org/10.1007/BF00260703

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