Summary
Autoradiographic studies of amino acid incorporation by Hymenolepis diminuta indicate high levels of protein synthetic activity in the subcuticular integumentary cells. Two hours after receiving a 5 minute pulse of tritiated proline, a significant percentage of the radioactivity initially incorporated into the subcuticular cytoplasm appears in the cuticular matrix. The results suggest that structural and non-mitochondrial enzymic proteins of the cuticle are synthesized in the subcuticular cells then secreted into the cuticular matrix. This interpretation is consistent with the cytology of the cestode integument revealed by electron microscopy.
Zusammenfassung
Autoradiographische Studien mit Aminosäuren, die Hymenolepis diminuta inkorporiert wurden, zeigen den hohen Grad der Protein-Syntheseaktivität in den Zellen des subcuticulären Integuments an. Zwei Stunden nach Applikation von tritiummarkiertem Prolin tritt in der cuticularen Matrix ein bedeutender Anteil der radioaktiven Substanz in Erscheinung. Die Resultate lassen vermuten, daß den subcuticulären Zellen strukturelle und enzymatische Proteine der Cuticula synthetisiert werden und dann in die cuticulare Matrix gelangen. Diese Interpretation steht in Übereinstimmung mit den cytologischen Befunden am Cestodenintegument, wie sie durch die Elktronenmikroskopie gewonnen wurden.
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This study was supported in part by grants from the National Institutes of Health, U.S. Public Health Service (5 TI AI 106 and AI 01384) and the American Cancer Society (IN-24-G). Portions of this investigation were conducted under the tenure of a predoctoral fellowship (1-Fl-GM-23, 449) from the U.S. Public Health Service.
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Lumsden, R.D. Cytological studies on the absorptive surfaces of cestodes. Z. F. Parasitenkunde 28, 1–13 (1966). https://doi.org/10.1007/BF00260524
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DOI: https://doi.org/10.1007/BF00260524