Summary
The fimD gene of Escherichia coli K12 was shown to be necessary for surface localization of type 1 fimbriae, since deletion of the gene resulted in a virtually bald phenotype. The FimD protein was found to be located in the outer membrane. Expressed alone, this protein had a very deleterious effect on cell growth. The DNA sequence of the fimD gene was determined; the corresponding amino acid sequence of the FimD protein was compared with those of the PapC and FaeD proteins. A deletion derivative of FimD gave clues as to which parts of the protein were necessary for outer membrane integration.
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References
Boyer HW, Roulland-Dussoix D (1969) A complementary analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Klemm P (1984) The fimA gene encoding the type 1 fimbrial subunit of Escherichia coli: nucleotide sequence and primary structure of the protein. Eur J Biochem 143:395–399
Klemm P (1985) Fimbrial adhesins of Escherichia coli. Rev Infect Dis 7:321–339
Klemm P, Christiansen G (1987) Three fim genes required for the regulation of length and mediation of adhesion of Escherichia coli Type 1 fimbriae. Mol Gen Genet 208:439–445
Klemm P, Jørgensen BJ, van Die I, de Ree H, Bergmans H (1985) The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli. Mol Gen Genet 199:410–414
Krogfelt KA, Klemm P (1988) Investigation of minor components of Escherichia coli Type 1 fimbriae: protein chemical and immunological aspects. Microbial Pathog 4:231–238
Mooi FR, Claassen I, Bakker D, Kuipers H, de Graaf FK (1986) Regulation and structure of an Escherichia coli gene coding for an outer membrane protein involved in export of K88ab fimbrial subunits. Nucleic Acids Res 14:2443–2457
Norgren M, Båga M, Tennent JM, Normark S (1987) Nucleotide sequence, regulation and functional analysis of the papC gene required for cell surface localization of Pap pili of uropathogenic Escherichia coli. Mol Microbiol 1:169–178
Oliver D (1985) Protein secretion in Escherichia coli. Annu Rev Microbiol 39:615–648
Orndorff PE, Falkow S (1984) Organization and expression of genes responsible for type 1 piliation in Escherichia coli. J Bacteriol 159:736–744
Osborn MJ, Gardner JE, Parin E, Carson J (1972) Mechanisms of assembly of the outer membrane of S. typhimurium. J. Biol Chem 247:3962–3972
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Thomson R, Achtman M (1978) The control region of the F factor DNA transfer cistrons: restriction mapping and DNA cloning. Mol Gen Genet 165:295–304
van Doorn J, Oudega B, Mooi FR, de Graaf FK (1982) Subcellular localization of polypeptides involved in the biosynthesis of K88ab fimbriae. FEMS Microbiol Lett 13:99–104
Volckaert G, Vleeschouwer E, Blöcker H, Frank R (1984) A novel type of cloning vectors for ultrarapid chemical degradation sequencing of DNA. Gene Anal Techn 1:52–59
von Heine G (1983) Patterns of amino acids near signal sequence cleavage sites. Eur J Biochem 133:17–21
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Communicated by J. Lengeler
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Klemm, P., Christiansen, G. The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae. Molec. Gen. Genet. 220, 334–338 (1990). https://doi.org/10.1007/BF00260505
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DOI: https://doi.org/10.1007/BF00260505