Summary
The gene of the major autolysin of Escherichia coli, the soluble lytic transglycosylase (Slt), was isolated from an expression gene library. The cloned slt gene was used to determine its chromosomal map position adjacent to trpR at 99.7 min on the E. coli linkage map.
This is a preview of subscription content, log in via an institution to check access.
References
Bachmann BJ (1983) Linkage map of Escherichia coli K12. Microbiol Rev 47:180–230
Cashel M, Rudd K (1987) The stringent response. In: Ingraham JL, Brooks Low K, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium. American Society for Microbiology, Washington, DC, pp 1410–1438
Gunsalus RP, Zurawski G, Yanowsky C (1979) Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr-region of the E. coli chromosome. J Bacteriol 140:106–113
Gutterson NI, Koshland DE (1983) Replacement and amplification of bacterial genes with sequences altered in vitro. Proc Natl Acad Sci USA 80:4894–4898
Hellman DM, Feramisco JR, Fiddes JC, Thomas GP, Hughes SH (1983) Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. Proc Natl Acad Sci USA 80:31–35
Hirota Y (1960) The effect of acridine dyes on mating type factors in Escherichia coli. Proc Natl Acad Sci USA 46:57–64
Höltje J-V, Schwarz U (1983) Coenzyme A-glutathione disulfide: An endogenous inhibitor of lytic enzymes in Escherichia coli. In: Hakenbeck R, Höltje J-V, Labischinski H (eds) The target of penicillin. Walter de Gryter, Berlin, New York, pp 185–190
Höltje J-V, Schwarz U (1985) Biosynthesis and growth of the murein sacculus. In: Nanninga N (ed) Molecular cytology of Escherichia coli. Academic Press, London, pp 77–119
Höltje J-V, Mirelman D, Sharon N, Schwarz U (1975) Novel type of murein transglycosylase in Escherichia coli. J Bacteriol 124:1067–1076
Kingsbury DT, Helinski DR (1973) Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: Requirement for deoxyribonucleic acid polymerase 1 in the replication of the plasmid ColE1. J Bacteriol 114:1116–1124
Kitano K, Tuomanen E, Tomasz A (1986) Transglycosylase and endopeptidase participate in the degradation of murein during autolysis of Escherichia coli. J Bacteriol 167:759–765
Kohara Y, Akiyama K, Isono K (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508
Kusser W, Ishiguro EE (1985) Involvement of the relA gene in the autolysis of Escherichia coli induced by inhibitors of peptidoglycan biosynthesis. J Bacteriol 136:861–865
Kusser W, Schwarz U (1980) Escherichia coli murein transglycosylase. Purification by affinity chromatography and interaction with polynucleotides. Eur J Biochem 103:277–281
Larsen JEL, Gerdes K, Light J, Molin S (1984) Low-copy-number plasmid cloning vectors amplifiable by derepression of an inserted foreign promoter. Gene 28:45–54
Roeder W, Somerville RL (1979) Cloning of the trpR gene. Mol Gen Genet 176:361–368
Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Author information
Authors and Affiliations
Additional information
Communicated by J.W. Lengeler
Rights and permissions
About this article
Cite this article
Betzner, A.S., Keck, W. Molecular cloning, overexpression and mapping of the slt gene encoding the soluble lytic transglycosylase of Escherichia coli . Mol Gen Genet 219, 489–491 (1989). https://doi.org/10.1007/BF00259625
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00259625