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A genetic and molecular analysis of P-induced mutations at the glucose-6-phosphate dehydrogenase locus in Drosophila melanogaster

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Summary

We examined P factor induced mutations of the Zw gene of Drosophila melanogaster in order to learn more about the site specificity of such mutations. Approximately 70000 chromosomes were screened using a powerful positive selection scheme. As only two mutants were discovered, Zw is a “cold spot” for transposable element insertion. One mutation involved a complex P element associated chromosomal rearrangement which was used to define the orientation of the gene with respect to the centromere of the X chromosome. The second mutation was either a simple, non-dysgenically induced point mutation or a very unstable insertion.

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Author notes

  1. Debra Nero

    Present address: Centre for Molecular Biology and Medicine, Monash University, 3168, Clayton, Victoria, Australia

Authors and Affiliations

  1. Department of Genetics and Developmental, Bradfield Hall, Cornell University, 14853, Ithaca, NY, USA

    Debra Nero, Nathaniel Bowditch III, Sally Pickert & Ross J. MacIntyre

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  1. Debra Nero
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  2. Nathaniel Bowditch III
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  3. Sally Pickert
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  4. Ross J. MacIntyre
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Communicated by B.H. Judd

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Nero, D., Bowditch, N., Pickert, S. et al. A genetic and molecular analysis of P-induced mutations at the glucose-6-phosphate dehydrogenase locus in Drosophila melanogaster . Mol Gen Genet 219, 429–438 (1989). https://doi.org/10.1007/BF00259616

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  • DOI: https://doi.org/10.1007/BF00259616

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