Skip to main content

A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants

Molecular and General Genetics MGG volume 219, pages 390–396 (1989)Cite this article

Summary

A new member of the patatin gene family belonging to the class II subfamily was isolated and characterized by DNA sequencing. In order to study the expression profile of this gene, the promoter was fused to the β-glucuronidase gene and transferred to potato and tobacco. Histochemical analysis revealed high expression in a few defined cells in potato tubers and in a specific layer of both potato and tobacco root tips. In contrast to the developmentally and metabolically regulated class I patatin gene B33 this gene was not inducible by elevated levels of sucrose. Expression of this chimaeric gene was also found in callus and suspension cultures of potato.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:8711–8721

    Google Scholar 

  • Bevan M, Barnes WM, Chilton M-D (1983) Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res 11: 369–385

    Google Scholar 

  • Bevan M, Barker R, Goldsbrough A, Jarvis M, Kavanagh T, Iturriaga G (1986) The structure and transcription start site of a major potato tuber protein gene. Nucleic Acids Res 14:4625–4638

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Google Scholar 

  • Dean C, van den Elzen P, Tamaki S, Dunsmuir P, Bedbrook J (1985) Differential expression of the eight genes of petunia ribulose bisphosphate carboxylase small subunit multi-gene family. EMBO J 4:3055–3061

    Google Scholar 

  • Dean C, Favreau M, Dunsmuir P, Bedbrook J (1987) Confirmation of the relative expression levels of the Petunia (Mitchell) rbcS genes. Nucleic Acids Res 15:4655–4668

    Google Scholar 

  • Esau K (1969) Pflanzenanatomie. Gustav Fischer Verlag, Stuttgart

    Google Scholar 

  • Goldberg RB (1988) Plants: novel developmental processes. Science 240:1460–1467

    Google Scholar 

  • Goldberg RB, Barker SJ, Perez-Grau L (1989) Gene expression during plant embryogenesis. Cell 56:149–160

    Google Scholar 

  • Hayward HE (1948) The structure of economic plants. The Macmillan Company, New York, pp 514–549

    Google Scholar 

  • Heidecker G, Messing J (1986) Structural analysis of plant genes. Annu Rev Plant Physiol 37:439–466

    Google Scholar 

  • Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405

    Google Scholar 

  • Jefferson R, Kavanagh TA, Bevan M (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, NY

    Google Scholar 

  • Mignery GA, Pikaard CS, Park WD (1988) Molecular characterization of the patatin multigene family of potato. Gene 62:27–44

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Google Scholar 

  • Nielsen NC, Dickinson CD, Cho T, Thanh VH, Scallon BJ, Fischer RL, Sims TL, Drews GN, Goldberg RB (1989) Characterization of the glycinin gene family in soybean. Plant Cell 1:313–328

    Google Scholar 

  • Pikaard CS, Mignery GA, Ma DP, Stark VJ, Park WD (1986) Sequence of two apparent pseudogenes of the major potato tuber protein, patatin. Nucleic Acids Res 14:5564–5566

    Google Scholar 

  • Pikaard CS, Brusca JS, Hannapel DJ, Park WD (1987) The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots. Nucleic Acids Res 15:1979–1994

    Google Scholar 

  • Racusen D, Foote M (1980) A major soluble glycoprotein of potato tubers. J Food Biochem 4:43–52

    Google Scholar 

  • Rocha-Sosa M, Sonnewald U, Frommer W, Stratmann M, Schell J, Willmitzer L (1989) Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J 8:23–29

    Google Scholar 

  • Rosahl S, Eckes P, Schell J, Willmitzer L (1986a) Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers. Mot Gen Genet 203:214–220

    Google Scholar 

  • Rosahl S, Schmidt R, Schell J, Willmitzer L (1986b) Organ-specific expression in potato: isolation and characterization of tuber-specific cDNA sequences. Mot Gen Genet 202:368–373

    Google Scholar 

  • Rosahl S, Schell J, Willmitzer L (1987) Expression of a tuber specific storage protein in transgenic tobacco plants: demonstration of an esterase activity. EMBO J 6:1155–1159

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Twell D, Ooms G (1987) The 5′ flanking DNA of a patatin gene directs tuber specific expression of a chimaeric gene in potato. Plant Mol Biol 9:345–375

    Google Scholar 

  • Twell D, Ooms G (1988) Structural diversity of the patatin gene family in potato cv. Desiree. Mol Gen Genet 212:325–336

    Google Scholar 

  • Wenzler HC, Mignery GA, Fisher LM, Park WD (1989) Analysis of a chimaeric class-I patatin-GUS gene in transgenic potato plants: high level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants. Plant Mol Biol 12:41–50

    Google Scholar 

  • Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103–119

    Google Scholar 

Download references

Author information

Author notes

  1. Mario Rocha-Sosa

    Present address: Centro de Investigation sobre Fijacion de Nitrogeno, Cuernavaca, Morelos, Mexico

  2. Sabine Rosahl, Jeff Schell & Lothar Willmitzer

    Present address: Max-Planck-Institut für Züchtungsforschung, Egelspfad, D-5000, Köln 30, Germany

Affiliations

  1. Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany

    Meike Köster-Töpfer, Wolf B. Frommer, Mario Rocha-Sosa, Sabine Rosahl, Jeff Schell & Lothar Willmitzer

Authors
  1. Meike Köster-Töpfer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wolf B. Frommer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mario Rocha-Sosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sabine Rosahl
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jeff Schell
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lothar Willmitzer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Saedler

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Köster-Töpfer, M., Frommer, W.B., Rocha-Sosa, M. et al. A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants. Mol Gen Genet 219, 390–396 (1989). https://doi.org/10.1007/BF00259611

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00259611

Key words

  • Class II patatin gene
  • β-glucuronidase
  • Transgenic potato
  • Transgenic tobacco
  • Root tip