Summary
Sporozoites, obtained from oocyst cultures stored at 4° C for more than 65 days were markedly less viable than those hatched from new oocyst cultures. There appeared to be no quantitative differences in the viability of sporozoites stored in liquid N2 for 99 days. Counts of macroscopic focal lesions on the chorioallantois of embryos infected with E. tenella appears to be an accurate method for assessing the viability of sporozoites of this species.
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References
Bovarnick, M. R., Miller, J. C., Snyder, J. C.: The influence of certain salts, amino acids, sugars and proteins on the stability of Rickettsiae. J. Bact. 59, 509–522 (1950).
Long, P. L.: Eimeria tenella: Chemotherapeutic studies in chick embryos with a description of a new method (chorioallantoic membrane foci counts) for evaluating infections. Z. Parasitenk. 33, 329–338 (1970).
Vetterling, J. M., Doran, D. J.: Storage polysaccharide in coccidial sporozoites after excystation and penetration of cells. J. Protozool. 16, 772–775 (1969).
Wagenbach, G. E., Challey, J. R., Burns, W. C.: A method for purifying coccidian oocysts employing Clorox and sulfuric acid-dichromate solution. J. Parasit. 52, 1222 (1966).
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Long, P.L. Studies on the viability of sporozoites of Eimeria tenella . Z. F. Parasitenkunde 35, 1–6 (1970). https://doi.org/10.1007/BF00259525
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DOI: https://doi.org/10.1007/BF00259525