Summary
The Ds-induced unstable sh-m6258 allele is caused by the insertion of at least 45 kb of non-sh DNA. Genomic clones spanning the two junctions of sh sequences with insert sequences were isolated and analysed. The long insert in the sh-m6258 allele is bordered by Ds sequences on either side. A 3 kb double Ds structure which consists of one complete Ds element of approximately 2 kb and one half Ds element of approximately 1 kb was found in the sh-m6258 allele at the junction between the 3′ region of the sh locus and the insert. At the junction between the 5′ region of the sh locus and the insert a half Ds element is present. This truncated Ds element was probably left behind after the excision of a 2 kb Ds element from a 3 kb double Ds structure similar to that found at the 3′ junction. Sequence analysis of the insert sequences beyond the Ds elements demonstrated that the long inserts in both the sh-m6258 and the sh-m5933 alleles originated from the same contiguous chromosomal segment in their common progenitor strain. One revertant derivative of the sh-m6258 allele was investigated. In the revertant strain, which displays a normal non-shrunken phenotype, a 2 kb Ds element is present at the site of the 45 kb insert in the mutant allele. This 2 kb insertion, which is located in the last but one intervening sequence of the Sh gene, does not inhibit expression of the gene. The 2 kb Ds element in the revertant allele is identical to the 2 kb Ds element which forms part of the 3 kb double Ds structure on the 3′ side of the insert of the sh-m6258 allele. We propose a hypothetical insert structure for the progenitor allele of the revertant. An intramolecular recombination or an unequal crossing-over event in this insert structure would account for the presence of a 2 kb Ds element in the revertant allele.
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References
Chourey PS, Nelson OE (1976) The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem Genet 14:1041–1055
Collins M, Rubin GM (1984) Structure of chromosomal rearrangements induced by the FB transposable element in Drosophila. Nature 308:323–327
Coupland G, Plum C, Chatterjee S, Post A, Starlinger P (1989) Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants. Proc Natl Acad Sci USA 86:9385–9388
Courage U, Döring H-P, Frommer W-B, Kunze R, Laird A, Merckelbach A, Müller-Neumann M, Riegel J, Starlinger P, Tillmann E, Weck E, Werr W, Yoder J (1984) Transposable elements Ac and Ds at the Shrunken, Waxy and Alcohol Dehydrogenase 1 Loci in Zea mays L. Cold Spring Harbor Symp Quant Biol 49:329–338
Courage-Tebbe U, Döring H-P, Fedoroff N, Starlinger P (1983) The controlling element Ds at the Shrunken locus in Zea mays: Structure of the unstable sh-m5933 allele and several revertants. Cell 34:383–393
Döring H-P, Starlinger P (1986) Molecular genetics of transposable elements in plants. Annu Rev Genet 20:175–200
Döring H-P, Tillmann E, Starlinger P (1984) DNA sequence of the maize transposable element Dissociation. Nature 307:127–130
Döring H-P, Garber R, Nelsen B, Tillmann E (1985) Transposable element Ds and chromosomal rearrangements. In: Freeling M (ed) Plant genetics. Alan Liss, New York, pp 355–367
Döring H-P, Nelsen-Salz B, Garber R, Tillmann E (1989) Double Ds elements are involved in specific chromosome breakage. Mol Gen Genet 219:299–305
Fedoroff NV (1983) Controlling elements in maize. In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 1–63
Fedoroff NV (1989) Maize transposable elements. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology, Washington, D.C., pp 375–411
Fedoroff NV, Mauvais J, Chaleff D (1983) Molecular studies on mutations at the Shrunken locus caused by the controlling element Ds. J Mol Appl Genet 2:11–29
Geiser M, Weck E, Döring H-P, Werr W, Courage-Tebbe U, Tillmann E, Starlinger P (1982) Genomic clones of a wild-type allele and a transposable element-induced mutant allele of the sucrose synthase gene of Zea mays L. EMBO J 1:1455–1460
Goldberg ML, Sheen J-Y, Gehring WJ, Green MM (1983) Unequal crossing-over associated with asymmetrical synapsis between nomadic elements in the Drosophila melanogaster genome. Proc Nail Acad Sci USA 80:5017–5021
Heinrich P (1985) Leitfaden zum schnellen and einfachen Plasmid-Sequenzieren. Copyright and ed. by Peter Heinrich
Klein A, Clancy M, Paje-Manalo L, Furtek DB, Hannah LC, Nelson OE Jr (1988) The mutation bronze-mutable 4 derivative 6856 in maize is caused by the insertion of a novel 6.7-kilobase pair transposon in the untranslated leader region of the bronze-1 gene. Genetics 120:779–790
Korfhage C, Döring H-P (1990) Chromosome breakage at the Dsinduced sh-m6233 allele. Maize Genet Coop News Lett 64:42
Kunze R, Stochaj U, Laufs J, Starlinger P (1987) Transcription of transposable element Activator (Ac) of Zea mays L. EMBO J 6:1555–1563
Martin C, MacKay S, Carpenter R (1988) Large-scale chromosomal restructuring is induced by the transposable element Tam3 at the nivea locus of Antirrhinum majus. Genetics 119:171–184
Maxam A, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
McClintock B (1946) Maize genetics. Carnegie Inst Wash Yearbook 45:176–186
McClintock B (1947) Cytogenetic studies of maize and neurospora. Carnegie Inst Wash Yearbook 46:146–152
McClintock B (1948) Mutable loci in maize. Carnegie Inst Wash Yearbook 47:155–169
McClintock B (1949) Mutable loci in maize. Carnegie Inst Wash Yearbook 48:142–154
McClintock B (1950) Mutable loci in maize. Carnegie Inst Wash Yearbook 49:157–167
McClintock B (1951) Chromosome organisation and genic expression. Cold Spring Harbor Symp Quant Biol 16:13–47
McClintock B (1952) Mutable loci in maize. Carnegie Inst Wash Yearbook 51:212–219
McClintock B (1953) Mutations in maize. Carnegie Inst Wash Yearbook 52:227–237
Mottinger JP (1970) The effects of x-rays on the bronze and shrunken loci in maize. Genetics 64:259–271
Murray NE (1983) Phage lambda and molecular cloning. In: Hendrix RW, Roberts JW, Stahl FW, Weisberg RA (eds) Lambda II. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 395–432
Nevers P, Shepherd NS, Saedler H (1986) Plant transposable elements. Adv Bot Res 12:103–203
Peterson PA (1987) Mobile elements in plants. CRC Crit Rev Plant Sci 6:105–208
Piatkowski D, Döring H-P (1989) Chromosome breakage at the Ds-induced sh-m6233-Dip allele. Maize Genet Coop News Lett 63:60–61
Sachs MM, Peacock WJ, Dennis ES, Gerlach WS (1983) Maize Ac/Ds controlling elements, a molecular viewpoint. Maydica 28:289–302
Saedler H, Nevers P (1985) Transposition in plants: a molecular model. EMBO J 4:585–590
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Weck E, Courage U, Döring H-P, Fedoroff N, Starlinger P (1984) Analysis of sh-m6233, a mutation induced by the transposable element Ds in the sucrose synthase gene of Zea mays. EMBO J 3:1713–1716
Werr W, Frommer W-B, Maas C, Starlinger P (1985) Structure of the sucrose synthase gene on chromosome 9 of Zea mays L. EMBO J 4:1373–1380
Wienand U, Feix G (1978) Electrophoretic fractionation and translation in vitro of poly(rA)-containing RNA from maize endosperm. Eur J Biochem 92:605–611
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Döring, H.P., Pahl, I. & Durany, M. Chromosomal rearrangements caused by the aberrant transposition of double Ds elements are formed by Ds and adjacent non-Ds sequences. Molec. Gen. Genet. 224, 40–48 (1990). https://doi.org/10.1007/BF00259449
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DOI: https://doi.org/10.1007/BF00259449