Summary
Three ATPase subunit 6 (atp6) genes of rice mitochondria were isolated, one from normal and two from cms-Bo male-sterile cytoplasms, in order to determine whether the extra atp6 gene in cms-Bo rice plays a role in cytoplasmic male-sterility (CMS). The nucleotide sequences of all three genes were determined and analysis showed a chimeric atp6 gene (urf-rmc) as well as a normal atp6 gene in cms-Bo cytoplasm, but only the normal atp6 gene in normal cytoplasm. The urf-rmc gene is completely homologous to the normal atp6 gene from at least position − 426 in the 5′ flanking region to position + 511 downstream from the initiation codon ATG; however, the following downstream sequence shows no homology with the normal rice atp6 gene, or any other reported sequence. Introduction of the restorer of fertility gene altered transcription of the urf-rmc gene but not the atp6 gene, indicating participation of the chimeric gene in the expression of CMS. Southern blot analysis showed that the urf-rmc gene was generated by an intramolecular recombination event in mitochondrial DNA, and the homologous recombination point between the atp6 gene and the opposite ancestral sequence was identified as 5′-TTCCCTC-3′.
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Communicated by R.G. Herrmann
The sequences reported in this paper have been deposited in the EMBL Data Library under accession numbers as follows: X52160, Oryza sativa mitochondrial urf-rmc gene (clone pOSB945X); X52161, O. sativa mitochondrial atp6 gene (clone pOSB1139); X52162, O. sativa mitochondrial atp6 gene (clone pOST558); X52163, O. sativa mitochondrial DNA (clone pOST642)
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Kadowaki, Ki., Suzuki, T. & Kazama, S. A chimeric gene containing the 5′ portion of atp6 is associated with cytoplasmic male-sterility of rice. Molec. Gen. Genet. 224, 10–16 (1990). https://doi.org/10.1007/BF00259445
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DOI: https://doi.org/10.1007/BF00259445