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N-Hydroxylation of 2-aminofluorene in the guinea pig and by guinea pig liver microsomes in vitro

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Summary

N-hydroxy-2-aminofluorene was found in the urine of guinea pigs intraperitoneally injected with 2-aminofluorene. The hydroxylamine was oxidized to the nitroso analogue and this was identified and determined in the carbon tetrachloride extract by its characteristic UV absorption, by thin-layer chromatography, and by the formation of a diazo compound in the reaction with nitrous acid. Only a small fraction of the 2-aminofluorene injected appeared in the urine as N-hydroxy derivative.

Guinea pig liver microsomes were observed to N-hydroxylate 2-aminofluorene rather rapidly, the reaction proceeding at least as rapidly as the N-hydroxylation of aniline.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität München, Germany

    Manfred Kiese, Gerhard Renner & Ingrid Wiedemann

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  1. Manfred Kiese
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  2. Gerhard Renner
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  3. Ingrid Wiedemann
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Kiese, M., Renner, G. & Wiedemann, I. N-Hydroxylation of 2-aminofluorene in the guinea pig and by guinea pig liver microsomes in vitro. Naunyn - Schmiedebergs Arch 252, 418–423 (1966). https://doi.org/10.1007/BF00258640

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  • DOI: https://doi.org/10.1007/BF00258640

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